Unitat d'Histologia i Neurobiologia (UHN), Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili, Reus, Spain.
J Peripher Nerv Syst. 2012 Sep;17(3):312-23. doi: 10.1111/j.1529-8027.2012.00419.x.
It has been shown that ciliary neurotrophic factor (CNTF) has trophic and maintenance effects on several types of peripheral and central neurons, glia, and cells outside the nervous system. Both CNTF and its receptor, CNTF-Rα, are expressed in the muscle. We use confocal immunocytochemistry to show that the trophic cytokine and its receptor are present in the pre- and post-synaptic sites of the neuromuscular junctions (NMJs). Applied CNTF (7.5-200 ng/ml, 60 min-3 h) does not acutely affect spontaneous potentials (size or frequency) or quantal content of the evoked acetylcholine release from post-natal (in weak or strong axonal inputs on dually innervated end plates or in the most mature singly innervated synapses at P6) or adult (P30) NMJ of Levator auris longus muscle of the mice. However, CNTF reduces roughly 50% the depression produced by repetitive stimulation (40 Hz, 2 min) on the adult NMJs. Our findings indicate that, unlike neurotrophins, exogenous CNTF does not acutely modulate transmitter release locally at the mammalian neuromuscular synapse but can protect mature end plates from activity-induced synaptic depression.
已证实睫状神经营养因子（CNTF）对多种外周和中枢神经元、神经胶质细胞以及神经系统外的细胞具有营养和维持作用。CNTF 和其受体 CNTF-Rα 均在肌肉中表达。我们使用共聚焦免疫细胞化学显示，这种营养细胞因子及其受体存在于神经肌肉接头（NMJ）的前突触和后突触部位。应用 CNTF（7.5-200ng/ml，60min-3h）不会急性影响出生后（在双神经支配终板上的弱或强轴突传入或 P6 时最成熟的单神经支配突触）或成年（P30）小鼠镫骨肌 Levator auris longus 肌肉 NMJ 的自发性电位（幅度或频率）或诱发乙酰胆碱释放的量子含量。然而，CNTF 可使成年 NMJ 上重复刺激（40Hz，2min）产生的抑制减少约 50%。我们的发现表明，与神经营养因子不同，外源性 CNTF 不会在哺乳动物神经肌肉突触处急性调节递质释放，而是可以保护成熟的终板免受活动诱导的突触抑制。
