Dept. Chemistry and Centre for Plastic Electronics, Imperial College London, Exhibition Road South Kensington, London SW7 2AY, UK.
Lab Chip. 2012 Nov 7;12(21):4313-20. doi: 10.1039/c2lc40718c.
We report the fabrication of non-emissive short- and long-pass filters on plastic for high sensitivity fluorescence detection. The filters were prepared by overnight immersion of titania-coated polyethylene terephthalate (PET) in an appropriate dye solution - xylene cyanol for short-pass filtering and fluorescein disodium salt for long-pass filtering - followed by repeated washing to remove excess dye. The interface between the titania and the dye molecule induces efficient quenching of photo-generated excitons in the dye molecule, reducing auto-fluorescence to negligible values and so overcoming the principal weakness of conventional colour filters. Using the filters in conjunction with a 505 nm cyan light-emitting diode and a Si photodiode, dose-response measurements were made for T8661 Transfluosphere beads in the concentration range 1 × 10(9) to 1 × 10(5) beads μL(-1), yielding a limit of detection of 3 × 10(4) beads μL(-1). The LED/short-pass filter/T8661/long-pass filter/Si-photodiode combination reported here offers an attractive solution for sensitive, low cost fluorescence detection that is readily applicable to a wide range of bead-based immunodiagnostic assays.
我们报告了在塑料上制造非发光短通和长通滤光片，用于高灵敏度荧光检测。滤光片通过将涂有二氧化钛的聚对苯二甲酸乙二醇酯（PET）在适当的染料溶液（二甲氧基荧光素用于短通过滤，荧光素二钠盐用于长通过滤）中浸泡一整夜来制备，然后反复洗涤以去除多余的染料。二氧化钛和染料分子之间的界面诱导染料分子中光生激子的有效猝灭，将自发荧光降低到可忽略的水平，从而克服了传统彩色滤光片的主要弱点。使用滤光片与 505nm 青绿色发光二极管和硅光电二极管一起，对浓度范围为 1×10(9)至 1×10(5)个珠子 μL(-1)的 T8661 Transfluosphere 珠子进行剂量响应测量，检测限为 3×10(4)个珠子 μL(-1)。这里报道的 LED/短通滤光片/T8661/长通滤光片/硅光电二极管组合为敏感、低成本荧光检测提供了有吸引力的解决方案，易于应用于广泛的基于珠子的免疫诊断测定。
