Pun R, Shah Y, Gupta G P, Sherchand S P, Pandey B D
Everest International Clinic and Research Centre, Kathmandu, Nepal.
Kathmandu Univ Med J (KUMJ). 2012 Jan-Mar;10(37):7-10. doi: 10.3126/kumj.v10i1.6905.
Dengue is an emerging vector borne disease in Nepal and rapid diagnostic test is important for early diagnosis of the disease.
The aim of the study was to evaluate the diagnostic accuracy of commonly used rapid immunochromatographic test kit in Nepal during 2010 dengue epidemic and to assess disease burden of dengue.
A total of 131 acute and nonacute serum samples were collected during recent epidemic of dengue in 2010 from clinically suspected Nepalese patients of different hospitals. Rapid immunochromatographic test kit was used for early diagnosis and enzyme immunosorbent was chosen as a reference assay.
The sensitivity and specificity of rapid test was 70% and 76.54% respectively whereas the prevalence of the disease was 38.17%. The odds ratio for males was 1.8 however; the association with the disease was statistically not significant.
The diagnostic accuracy of rapid immunochromatographic test for dengue diagnosis was low (k=0.46). So, it should be substituted by highly sensitive test device for prompt diagnosis and health personnel should consider appropriate timing of sample collection for better performance of rapid test.
登革热是尼泊尔一种新出现的媒介传播疾病，快速诊断检测对于该疾病的早期诊断很重要。
本研究的目的是评估2010年登革热疫情期间尼泊尔常用的快速免疫层析检测试剂盒的诊断准确性，并评估登革热的疾病负担。
2010年近期登革热疫情期间，从不同医院临床疑似的尼泊尔患者中总共收集了131份急性和非急性血清样本。使用快速免疫层析检测试剂盒进行早期诊断，并选择酶联免疫吸附测定作为参考检测方法。
快速检测的灵敏度和特异性分别为70%和76.54%，而该疾病的患病率为38.17%。男性的优势比为1.8；然而，与该疾病的关联在统计学上不显著。
登革热诊断的快速免疫层析检测的诊断准确性较低（κ=0.46）。因此，应使用高灵敏度检测设备替代它以进行快速诊断，并且卫生人员应考虑适当的样本采集时间，以使快速检测有更好的表现。
