Shih Hsin-I, Hsu Hsiang-Chin, Wu Chi-Jung, Lin Chih-Hao, Chang Chia-Ming, Tu Yi-Fang, Hsieh Chih-Chia, Chi Chih-Hsien, Sung Tzu-Ching
Department of Emergency Medicine, National Cheng Kung University Hospital, National Cheng Kung University Tainan, Taiwan.
Department of Emergency Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
PLoS One. 2016 Jul 14;11(7):e0158437. doi: 10.1371/journal.pone.0158437. eCollection 2016.
Dengue infection is a major health problem in tropical and subtropical countries. A prospective observational study in a university-affiliated hospital was conducted between August 2015 and September 2015. Patients who visited the emergency department (ED) with a presentation of any symptoms of dengue were eligible for the dengue non-structural protein 1 (NS1), IgM/IgG rapid immunochromatographic tests and real-time polymerase chain reaction (RT-PCR) to evaluate the performance of the rapid tests. Considering the RT-PCR as the gold standard for the dengue diagnosis, the ideal primary results of sensitivity (80-100%), specificity (60-84%), positive predicted value(75%-95%), and negative predicted value (70-100%) suggested that the NS1-based test with or without a combination of IgM and IgG tests have good diagnostic performances in detecting dengue infections, even in the afebrile or elderly populations.
登革热感染是热带和亚热带国家的一个主要健康问题。2015年8月至2015年9月期间，在一家大学附属医院进行了一项前瞻性观察研究。因出现任何登革热症状而前往急诊科就诊的患者，有资格接受登革热非结构蛋白1（NS1）、IgM/IgG快速免疫层析试验和实时聚合酶链反应（RT-PCR），以评估快速检测的性能。将RT-PCR视为登革热诊断的金标准，灵敏度（80%-100%）、特异性（60%-84%）、阳性预测值（75%-95%）和阴性预测值（70%-100%）的理想初步结果表明，基于NS1的检测无论是否结合IgM和IgG检测，在检测登革热感染方面都具有良好的诊断性能，即使在无发热或老年人群中也是如此。
