Division of Sport Science, College of Natural Sciences, Konkuk University, Chungbuk 380-702, Republic of Korea.
J Ethnopharmacol. 2011 Sep 1;137(1):802-7. doi: 10.1016/j.jep.2011.06.043. Epub 2011 Jul 5.
The Lindera obtusiloba has been used in traditional medicine for the treatment of inflammation and dermatitis. In this study, we investigated the effect of topical application of Lindera obtusiloba water extract (LOWE) on the house dust mite extract (Dermatophagoides farinae extract, DFE) and 2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis (AD).
We established AD model in BALB/c mice by repeated local exposure of DFE/DNCB to the ears. After a topical application of LOWE on the skin lesions, the epidermal thickness, mast cell infiltration, and serum immunoglobulin E (IgE) and histamine were measured. In addition, the gene expression of interleukin (IL)-4, IL-13, IL-31, and tumor necrosis factor (TNF)-α in the ears was assayed.
LOWE reduced AD symptoms based on ear thickness, histopathological analysis, and serum IgE levels. LOWE inhibited mast cell infiltration into the ear and elevation of serum histamine in AD model. Moreover, LOWE suppressed DFE/DNCB-induced expression of IL-4, IL-13, IL-31, and TNF-α in the ears.
Our results showed that topical application of LOWE exerts beneficial effects in AD symptoms, suggesting that LOWE might be a candidate for the treatment of AD.
钝叶乌药在传统医学中用于治疗炎症和皮炎。在这项研究中，我们研究了钝叶乌药水提取物（LOWE）局部应用于屋尘螨提取物（粉尘螨提取物，DFE）和 2,4-二硝基氯苯（DNCB）诱导的特应性皮炎（AD）的效果。
我们通过反复局部暴露 DFE/DNCB 于耳朵来建立 AD 模型。在皮肤损伤处涂抹 LOWE 后，测量表皮厚度、肥大细胞浸润以及血清免疫球蛋白 E（IgE）和组胺。此外，还测定了耳朵中白细胞介素（IL）-4、IL-13、IL-31 和肿瘤坏死因子（TNF）-α的基因表达。
基于耳朵厚度、组织病理学分析和血清 IgE 水平，LOWE 减轻了 AD 症状。LOWE 抑制了 AD 模型中肥大细胞浸润到耳朵和血清组胺的升高。此外，LOWE 抑制了 DFE/DNCB 诱导的耳朵中 IL-4、IL-13、IL-31 和 TNF-α的表达。
我们的结果表明，局部应用 LOWE 对 AD 症状有有益的影响，提示 LOWE 可能是 AD 治疗的候选药物。
