新型引物探针组合通过 PCR 微阵列分析检测和鉴定分枝杆菌。
Novel primer-probe sets for detection and identification of mycobacteria by PCR-microarray assay.
机构信息
Laboratorio di Microbiologia Molecolare e Biotecnologia, Dipartimento di Biotecnologie, Università di Siena, Siena, Italy.
出版信息
J Clin Microbiol. 2012 Nov;50(11):3777-9. doi: 10.1128/JCM.02300-12. Epub 2012 Sep 12.
Abstract
A PCR-microarray assay was developed in which PCR primers and hybridization probes were designed for the 16S rRNA genes of 16 clinically relevant mycobacteria and for IS6110 of Mycobacterium tuberculosis. The assay, based on a multiplex PCR followed by hybridization with oligonucleotide probes, was tested against 16 Mycobacterium species and 70 clinical samples.
摘要
开发了一种 PCR-微阵列分析方法，该方法针对 16 种临床相关分枝杆菌的 16S rRNA 基因和结核分枝杆菌的 IS6110 设计了 PCR 引物和杂交探针。该方法基于多重 PCR 后与寡核苷酸探针杂交，对 16 种分枝杆菌和 70 份临床样本进行了测试。
相似文献
1
Novel primer-probe sets for detection and identification of mycobacteria by PCR-microarray assay.新型引物探针组合通过 PCR 微阵列分析检测和鉴定分枝杆菌。
J Clin Microbiol. 2012 Nov;50(11):3777-9. doi: 10.1128/JCM.02300-12. Epub 2012 Sep 12.
2
Combination of multiplex PCR with denaturing high-performance liquid chromatography for rapid detection of Mycobacterium genus and simultaneous identification of the Mycobacterium tuberculosis complex.多重聚合酶链反应与变性高效液相色谱联用快速检测分枝杆菌属并同时鉴定结核分枝杆菌复合群。
Diagn Microbiol Infect Dis. 2013 Sep;77(1):53-7. doi: 10.1016/j.diagmicrobio.2013.06.003. Epub 2013 Jul 16.
3
Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex.用于区分牛分枝杆菌与结核分枝杆菌复合群的多重聚合酶链反应。
Braz J Microbiol. 2014 Oct 9;45(3):841-3. doi: 10.1590/s1517-83822014000300012. eCollection 2014.
4
Development and evaluation of a multiplex polymerase chain reaction for the detection of Mycobacterium tuberculosis from pulmonary specimens.用于从肺部标本中检测结核分枝杆菌的多重聚合酶链反应的开发与评估
Scand J Infect Dis. 2012 Oct;44(10):739-44. doi: 10.3109/00365548.2012.684219. Epub 2012 Jun 18.
5
Multiplex real-time PCR assay and melting curve analysis for identifying Mycobacterium tuberculosis complex and nontuberculous mycobacteria.多重实时 PCR 检测和熔解曲线分析用于鉴定结核分枝杆菌复合群和非结核分枝杆菌。
J Clin Microbiol. 2012 Feb;50(2):483-7. doi: 10.1128/JCM.06155-11. Epub 2011 Dec 7.
6
Russian "successful" clone B0/W148 of Mycobacterium tuberculosis Beijing genotype: a multiplex PCR assay for rapid detection and global screening.俄罗斯“成功”克隆的结核分枝杆菌北京基因型 B0/W148：一种快速检测和全球筛查的多重 PCR 检测方法。
J Clin Microbiol. 2012 Nov;50(11):3757-9. doi: 10.1128/JCM.02001-12. Epub 2012 Aug 29.
7
Validation of a Multiplex Real-Time PCR Assay for Detection of Mycobacterium spp., Mycobacterium tuberculosis Complex, and Mycobacterium avium Complex Directly from Clinical Samples by Use of the BD Max Open System.使用BD Max开放式系统直接从临床样本中检测分枝杆菌属、结核分枝杆菌复合群和鸟分枝杆菌复合群的多重实时PCR检测方法的验证
J Clin Microbiol. 2016 Jun;54(6):1644-1647. doi: 10.1128/JCM.00241-16. Epub 2016 Mar 23.
8
Role of multiplex polymerase chain reaction using IS6110 and Protein b for the diagnosis of extra-pulmonary tuberculosis: North India.使用IS6110和蛋白b的多重聚合酶链反应在肺外结核病诊断中的作用：印度北部
Indian J Pathol Microbiol. 2015 Jan-Mar;58(1):27-30. doi: 10.4103/0377-4929.151164.
9
Novel multiplex PCR using dual-priming oligonucleotides for detection and discrimination of the Mycobacterium tuberculosis complex and M. bovis BCG.基于双重引物寡核苷酸的新型多重 PCR 检测方法，用于结核分枝杆菌复合群和牛型结核分枝杆菌的检测和区分。
J Clin Microbiol. 2010 Dec;48(12):4612-4. doi: 10.1128/JCM.00872-10. Epub 2010 Oct 13.
10
Novel real-time simultaneous amplification and testing method to accurately and rapidly detect Mycobacterium tuberculosis complex.新型实时同步扩增检测方法，可准确快速检测结核分枝杆菌复合群。
J Clin Microbiol. 2012 Mar;50(3):646-50. doi: 10.1128/JCM.05853-11. Epub 2012 Jan 11.
引用本文的文献
1
A 69.9-kb long inverted repeat increases genome instability in a strain of .一个69.9千碱基对长的反向重复序列增加了某菌株中的基因组不稳定性。
NAR Genom Bioinform. 2025 Jun 26;7(2):lqaf085. doi: 10.1093/nargab/lqaf085. eCollection 2025 Jun.
2
A nisin-inducible chromosomal gene expression system based on ICE Tn5253 of Streptococcus pneumoniae, transferable among streptococci and enterococci.基于肺炎链球菌 ICE Tn5253 的乳链菌肽诱导型染色体基因表达系统，可在链球菌属和肠球菌属间转移。
World J Microbiol Biotechnol. 2024 Sep 12;40(10):319. doi: 10.1007/s11274-024-04124-6.
3
Solid and Suspension Microarrays for Microbial Diagnostics.用于微生物诊断的固相和悬浮微阵列
Methods Microbiol. 2015;42:395-431. doi: 10.1016/bs.mim.2015.04.002. Epub 2015 May 14.
4
Surgical site infections by atypical mycobacteria: prevalence and species characterization using MALDI-TOF and molecular LCD chip array.非典型分枝杆菌引起的手术部位感染：使用 MALDI-TOF 和分子 LCD 芯片阵列进行的流行率和种系特征分析。
Infection. 2022 Dec;50(6):1557-1563. doi: 10.1007/s15010-022-01864-1. Epub 2022 Jun 18.
5
Microfluidics as a Novel Technique for Tuberculosis: From Diagnostics to Drug Discovery.微流控技术作为结核病的一种新技术：从诊断到药物发现
Microorganisms. 2021 Nov 11;9(11):2330. doi: 10.3390/microorganisms9112330.
6
A pilot study: VereMTB detection kit for rapid detection of multidrug-resistant mycobcterium tuberculosis in clinical sputum samples.一项初步研究： VereMTB 检测试剂盒在临床痰液样本中快速检测耐多药结核分枝杆菌。
PLoS One. 2020 Mar 9;15(3):e0228312. doi: 10.1371/journal.pone.0228312. eCollection 2020.
7
Lab-on-Chip-Based Platform for Fast Molecular Diagnosis of Multidrug-Resistant Tuberculosis.基于芯片实验室的多药耐药结核病快速分子诊断平台。
J Clin Microbiol. 2015 Dec;53(12):3876-80. doi: 10.1128/JCM.01824-15. Epub 2015 Aug 5.
8
Rapid inactivation of Mycobacterium and nocardia species before identification using matrix-assisted laser desorption ionization-time of flight mass spectrometry.在使用基质辅助激光解吸电离飞行时间质谱进行鉴定之前对分枝杆菌和诺卡氏菌属菌种进行快速灭活。
J Clin Microbiol. 2014 Oct;52(10):3654-9. doi: 10.1128/JCM.01728-14. Epub 2014 Jul 30.
本文引用的文献
1
Rapid detection of Mycobacterium tuberculosis and rifampin resistance by use of on-demand, near-patient technology.采用按需、近患者技术快速检测结核分枝杆菌和利福平耐药性。
J Clin Microbiol. 2010 Jan;48(1):229-37. doi: 10.1128/JCM.01463-09. Epub 2009 Oct 28.
2
Rapid Identification of Mycobacterium tuberculosis and nontuberculous mycobacteria by multiplex, real-time PCR.通过多重实时聚合酶链反应快速鉴定结核分枝杆菌和非结核分枝杆菌
J Clin Microbiol. 2009 May;47(5):1497-502. doi: 10.1128/JCM.01868-08. Epub 2009 Mar 18.
3
Pulmonary infections associated with non-tuberculous mycobacteria in immunocompetent patients.免疫功能正常患者中与非结核分枝杆菌相关的肺部感染
Lancet Infect Dis. 2008 May;8(5):323-34. doi: 10.1016/S1473-3099(08)70100-2.
4
DNA microarray for detection of macrolide resistance genes.用于检测大环内酯类耐药基因的DNA微阵列
Antimicrob Agents Chemother. 2006 Jun;50(6):2038-41. doi: 10.1128/AAC.01574-05.
5
Detection and identification of Mycobacterium species isolates by DNA microarray.通过DNA微阵列检测和鉴定分枝杆菌属分离株
J Clin Microbiol. 2003 Jun;41(6):2605-15. doi: 10.1128/JCM.41.6.2605-2615.2003.
6
Clinical and taxonomic status of pathogenic nonpigmented or late-pigmenting rapidly growing mycobacteria.致病性非色素沉着或迟色素沉着快速生长分枝杆菌的临床和分类学地位
Clin Microbiol Rev. 2002 Oct;15(4):716-46. doi: 10.1128/CMR.15.4.716-746.2002.
7
The rapidly growing mycobacteria: saprophytes and parasites.快速生长的分枝杆菌：腐生菌和寄生菌。
Microbes Infect. 2000 Dec;2(15):1845-53. doi: 10.1016/s1286-4579(00)01338-1.
8
A unified view of polymer, dumbbell, and oligonucleotide DNA nearest-neighbor thermodynamics.聚合物、哑铃状分子和寡核苷酸DNA最近邻热力学的统一观点。
Proc Natl Acad Sci U S A. 1998 Feb 17;95(4):1460-5. doi: 10.1073/pnas.95.4.1460.
9
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.空位BLAST和位置特异性迭代BLAST：新一代蛋白质数据库搜索程序。
Nucleic Acids Res. 1997 Sep 1;25(17):3389-402. doi: 10.1093/nar/25.17.3389.
10
Epidemiology of infection by nontuberculous mycobacteria.非结核分枝杆菌感染的流行病学
Clin Microbiol Rev. 1996 Apr;9(2):177-215. doi: 10.1128/CMR.9.2.177.
Zotero 插件