俄罗斯“成功”克隆的结核分枝杆菌北京基因型 B0/W148：一种快速检测和全球筛查的多重 PCR 检测方法。
Russian "successful" clone B0/W148 of Mycobacterium tuberculosis Beijing genotype: a multiplex PCR assay for rapid detection and global screening.
机构信息
Laboratory of Molecular Microbiology, St. Petersburg Pasteur Institute, St. Petersburg, Russia.
出版信息
J Clin Microbiol. 2012 Nov;50(11):3757-9. doi: 10.1128/JCM.02001-12. Epub 2012 Aug 29.
Abstract
We describe a multiplex PCR assay to detect the Mycobacterium tuberculosis Beijing genotype variant B0/W148, which is considered a "successful" clone of M. tuberculosis, widespread in Russia. Specificity and sensitivity of the assay were 100% based on the analysis of a collection of 516 M. tuberculosis isolates of different genotypes and origins. This assay may be used for accurate and simple detection and surveillance of this clinically and epidemiologically important variant of M. tuberculosis.
摘要
我们描述了一种多重 PCR 检测方法，用于检测结核分枝杆菌北京基因型变异体 B0/W148，该变异体被认为是结核分枝杆菌的一种“成功”克隆，广泛存在于俄罗斯。基于对来自不同基因型和来源的 516 株结核分枝杆菌分离株的分析，该检测方法的特异性和敏感性均为 100%。该检测方法可用于结核分枝杆菌这一临床和流行病学重要变异体的准确、简单的检测和监测。
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