Infectious Diseases Programme, Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117597.
Evid Based Complement Alternat Med. 2012;2012:294528. doi: 10.1155/2012/294528. Epub 2012 Aug 29.
We provide the scientific basis for the use of Calotropis procera for treating skin and wound infections in traditional medicine. The aqueous extract of stem-bark of C. procera exhibited more pronounced potent antimicrobial activity. Calo-protein was purified and identified from the most-active aqueous extracts of C. procera and showed broad-spectrum activity. Calo-protein inhibited the growth of S. aureus and E. aerogenes effectively at 25 μg/ml concentration. Mice topically treated with Calo-protein revealed significant wound healing after 14 days comparable to fusidic acid (FA) as positive control. This protein was devoid of cytolytic effect even at higher concentrations on skin cells after 24 h. Further investigation of this Calo-protein of C. procera on bacterial inhibition may provide a better understanding of the scientific basis and justification for its use in traditional medicine.
我们为 calotropis procera 在传统医学中治疗皮肤和伤口感染提供了科学依据。茎皮的水提物表现出更明显的强大抗菌活性。从 calotropis procera 的最有效水提物中纯化并鉴定出 calo 蛋白，表现出广谱活性。Calo 蛋白在 25μg/ml 浓度下有效抑制金黄色葡萄球菌和产气肠杆菌的生长。经 calo 蛋白局部治疗的小鼠在 14 天后伤口愈合明显，与阳性对照的夫西地酸（FA）相当。这种蛋白即使在 24 小时后对皮肤细胞的浓度更高，也没有细胞毒性作用。进一步研究 calo 蛋白对细菌抑制的作用可能为其在传统医学中的应用提供更好的科学依据和解释。
