Tsala David Emery, Nga Nnanga, Thiery Bella Ndzana Martin, Bienvenue Mballa Therese, Theophile Dimo
Department of Biological Sciences, Faculty of Science, University of Maroua, Maroua-Cameroon.
Department of Pharmaceutical Sciences, Falculty of Medicine and Pharmaceutical Sciences, University of Douala, Yaounde, Cameroon/Institute of Medical Research and Medicinal Plants Studies (IMPM), Yaoundé, Cameroon/Département de Pharmacie galénique et Législation Pharmaceutique, Faculté de Médecine et des Sciences Biomédicales, Université de Yaounde I, Cameroun.
J Intercult Ethnopharmacol. 2015 Jan-Mar;4(1):64-9. doi: 10.5455/jice.20141211071136. Epub 2015 Jan 3.
The objective of the present study was to evaluate the antioxidant potential and the wound healing effect of the ethanolic extract of the bark of Calotropis procera. The antioxidant study was evaluated in vitro, using 2,2-diphenylpicrylhydrazyl (DPPH) and deoxyribose degradation assays. Wound healing was studied using excision and incision wound on normal and dexamethasone-suppressed wound healing rodent models. Alkaloids, flavonoids, proteins and phenols were screened in the extract used whereas saponins and true tannins were absent. The extract contains only 12.5 gallic acid equivalent and 399.54 rutin equivalent. It was found to inhibit DPPH and deoxyribose oxidation (IC50 = 24.24 and 5.40 respectively). In vivo study demonstrated a significant reduction in the epithelialization time (P < 0.001) to 17-18 days in normal and dexamethasone treated rats following the ethanolic extract of the bark of C. procera application. The same extract also significantly increased the breaking strength in dexamethasone treated rats. Histological examination of incision wounds of treated group showed matured extracellular matrix, numerous fibroblasts. This study illustrated an excellent potential of the bark of C. procera therapy on dermal wound healing, with a tentative mechanism of action related to improved collagen deposition and reduced inflammatory reaction.
本研究的目的是评估牛角瓜树皮乙醇提取物的抗氧化潜力和伤口愈合效果。抗氧化研究采用2,2-二苯基苦味酰基自由基（DPPH）和脱氧核糖降解试验在体外进行评估。使用切除和切开伤口模型，在正常和地塞米松抑制伤口愈合的啮齿动物模型上研究伤口愈合情况。对所用提取物中的生物碱、黄酮类化合物、蛋白质和酚类进行了筛选，未检测到皂苷和真单宁。该提取物仅含有12.5没食子酸当量和399.54芦丁当量。发现其能抑制DPPH和脱氧核糖氧化（IC50分别为24.24和5.40）。体内研究表明，在正常和地塞米松处理的大鼠中，应用牛角瓜树皮乙醇提取物后，上皮化时间显著缩短至17 - 18天（P < 0.001）。同一提取物还显著提高了地塞米松处理大鼠的抗张强度。治疗组切开伤口的组织学检查显示细胞外基质成熟，成纤维细胞众多。本研究表明牛角瓜树皮疗法在皮肤伤口愈合方面具有良好潜力，其作用机制初步认为与改善胶原蛋白沉积和减轻炎症反应有关。
