Department of Dermatology, Bundang Seoul National University Hospital, Seoul National University College of Medicine, 166 Gumi-ro, Bundang-gu, Seongnam-si 463-707, Republic of Korea.
Oxid Med Cell Longev. 2012;2012:819623. doi: 10.1155/2012/819623. Epub 2012 Aug 29.
The skin is constantly exposed to environmental oxidative stress. Skin equivalent (SE) models are three-dimensional systems in which cell-cell or cell-matrix interactions can be investigated. In this study, the effects of vitamin C or plant extracts with high antioxidant activities were tested. There was no significant difference in the epidermal thickness, but the basal cells became cuboidal when vitamin C or plant extracts were supplemented. Furthermore, immunohistochemical staining showed linear and intense staining of α6 and β1 integrin along the basement membrane in vitamin C or plant extract treated models. The p63 and PCNA were also stained. Results showed that the number of p63 and PCNA positive cells was higher in the vitamin C or plant extract treated models than in the control SEs. Although the relationship between oxidative stress and stem cells is not known, our results suggest that redox status affects the stemness and the proliferative potential of epidermal basal cells by modulating microenvironment to epidermal basal stem cells.
皮肤不断受到环境氧化应激的影响。皮肤等效物（SE）模型是一种三维系统，可以研究细胞-细胞或细胞-基质的相互作用。在这项研究中，测试了维生素 C 或具有高抗氧化活性的植物提取物的作用。表皮厚度没有明显差异，但当补充维生素 C 或植物提取物时，基底细胞变成了立方体形。此外，免疫组织化学染色显示，在维生素 C 或植物提取物处理的模型中，α6 和 β1 整联蛋白沿着基底膜呈线性和强烈染色。还染色了 p63 和 PCNA。结果表明，维生素 C 或植物提取物处理的模型中 p63 和 PCNA 阳性细胞的数量高于对照 SE。尽管氧化应激和干细胞之间的关系尚不清楚，但我们的结果表明，氧化还原状态通过调节微环境对表皮基底干细胞来影响表皮基底细胞的干性和增殖潜力。
