Laboratory of Marine Life Ecophysiology, Department of Oceanographic Biology, Institute of Oceanography, Universidade de São Paulo, Praça do Oceanográfico, 191 Cidade Universitária, São Paulo, SP, CEP 05508-900, Brazil.
Environ Toxicol Pharmacol. 2012 Nov;34(3):969-76. doi: 10.1016/j.etap.2012.07.010. Epub 2012 Jul 31.
In the present study, the polycyclic aromatic hydrocarbon (PAH) genotoxicity was investigated in a one-step predator-prey relationship with the trophic-related marine species. Florida pompanos were fed for 5 and 10 days with pink shrimp post larvae previously exposed to benzo(a)pyrene (BaP) concentrations. Parent BaP body burden was measured in samples of Farfantepenaeus brasiliensis. BaP metabolites were determined in bile samples of Trachinotus carolinus and DNA damage was assessed through the comet and erythrocyte nuclear abnormalities (ENAs) assays in fish erythrocytes. BaP body burden increased significantly with the PAH concentration in pink shrimp PLs as well as the fish bile BaP metabolites. Both, comet and ENAs assays indicated significant increase on erythrocyte DNA damage of Florida pompanos fed with BaP-exposed pink shrimp on both feeding periods. The trophic route of BaP genotoxicity is discussed as well as the PAH biotransformation as the inducing mechanism for the DNA damages observed.
在本研究中，通过研究与营养相关的海洋物种的一步式捕食者-被捕食关系，研究了多环芳烃（PAH）的遗传毒性。用先前暴露于苯并（a）芘（BaP）浓度的粉虾幼体喂养佛罗里达石斑鱼 5 和 10 天。测定了凡纳滨对虾体内的母体 BaP 体负荷。测定了卡罗来纳无须鲷的胆汁样本中的 BaP 代谢物，并通过彗星和红细胞核异常（ENAs）试验评估了鱼类红细胞中的 DNA 损伤。随着粉虾 PL 中的 PAH 浓度以及鱼类胆汁中 BaP 代谢物的增加，BaP 体负荷显著增加。在两种喂食期，喂食 BaP 暴露粉虾的佛罗里达石斑鱼的彗星和 ENAs 试验均表明红细胞 DNA 损伤显著增加。讨论了 BaP 遗传毒性的营养途径，以及作为观察到的 DNA 损伤诱导机制的 PAH 生物转化。
