Laboratório de Ecofisiologia de Animais Marinhos, Departamento de Oceanografia Biológica, Instituto Oceanográfico, Universidade de São Paulo, Praça do Oceanográfico, 191 Cidade Universitária, CEP 05508-900, São Paulo, Brazil.
Environ Toxicol Pharmacol. 2012 Nov;34(3):995-1003. doi: 10.1016/j.etap.2012.07.006. Epub 2012 Jul 31.
Seabob shrimp Xiphopenaeus kroyeri is a marine species that lives in shallow waters of coastal environments, often impacted by polycyclic aromatic hydrocarbons (PAH) pollution. In the present study, seabob shrimp were exposed for 96 h to benzo[a]pyrene (BaP) at the nominal concentrations of 100, 200, 400 and 800 microg-L(-1). Animals of the control groups were exposed either to clean water or to the BaP-carrier (DMSO). At the end of the exposures, muscle tissues were sampled for BaP uptake assessment and hepatopancreas and hemolymph for EROD enzyme activity and hemocytes DNA damage, respectively. EROD activity and DNA damage increased significantly as a function of BaP exposure concentrations. Significant correlations between BaP uptake and both EROD activity and DNA damage suggest that they can be used as suitable tools for integrated levels of study on the biomarkers of PAH exposure.
拟穴青蟹（Xiphopenaeus kroyeri）是一种生活在沿海浅水环境中的海洋物种，经常受到多环芳烃（PAH）污染的影响。在本研究中，拟穴青蟹在苯并[a]芘（BaP）的名义浓度为 100、200、400 和 800μg/L（-1）下暴露 96 h。对照组的动物要么暴露在清洁水中，要么暴露在 BaP 载体（DMSO）中。暴露结束时，分别从肌肉组织中取样以评估 BaP 的摄取情况，从肝胰腺和血淋巴中取样以评估 EROD 酶活性和血细胞 DNA 损伤。随着 BaP 暴露浓度的增加，EROD 活性和 DNA 损伤显著增加。BaP 摄取与 EROD 活性和 DNA 损伤之间存在显著相关性，表明它们可用作 PAH 暴露生物标志物综合水平研究的合适工具。
