Key Laboratory of Mariculture, Ministry of Education, Fisheries College, Ocean University of China, Yushan Road 5, Qingdao 266003, PR China.
Key Laboratory of Mariculture, Ministry of Education, Fisheries College, Ocean University of China, Yushan Road 5, Qingdao 266003, PR China.
Environ Toxicol Pharmacol. 2015 Jan;39(1):194-207. doi: 10.1016/j.etap.2014.08.006. Epub 2014 Aug 13.
The purpose of this study was to investigate the toxic effects induced by benzo[a]pyrene (BaP). In the present study, juvenile white shrimp Litopenaeus vannamei were exposed to BaP for 21 days at four different concentrations as 0, 0.03, 0.3 and 3μg/L. Detoxification system parameters, transcription of metabolism-related genes, and damage indexes were investigated for screening. It showed mRNA expression levels of superoxide dismutase (SOD), cytochrome P450 (CYP) 1A1 and glutathione-S-transferase (GST) in hepatopancreas were significantly induced at day 3 by 0.3 and 3μg/L BaP, and returned to the initial level afterwards. 7-Ethoxyresorufin O-deethylase (EROD), GST and SOD activities in gill and hepatopancreas were also induced by 0.3 and 3μg/L BaP, while T-AOC and GSH contents were reduced after BaP exposure. The study also showed that 0.3 and 3μg/L BaP caused damage effects, while 0.03μg/L BaP treatment did not exhibit any damage effects. Moreover, according to the correlation analysis results, we conclude that EROD activity, GST activity and GSH content in hepatopancreas can be used as defense biomarkers; DNA strand breaks, lipid peroxidation (LPO) level and protein carbonyl (PC) content in gill and hepatopancreas can be used as damage biomarkers; EROD activity, GST activity, GSH contents and damage effects of DNA strand breaks, LPO level and PC content in hepatopancreas can be used as combined biomarkers. These results will provide information not only on potential biomarkers that could be effectively applied to biomonitor aquatic environment to contamination, but also provide information on toxic effects of this specie.
本研究旨在探讨苯并[a]芘（BaP）引起的毒性作用。在本研究中，将幼白对虾（Litopenaeus vannamei）暴露于 0、0.03、0.3 和 3μg/L 四种不同浓度的 BaP 中 21 天。调查解毒系统参数、代谢相关基因的转录和损伤指标，以进行筛选。结果表明，在 0.3 和 3μg/L BaP 作用下，肝胰腺中超氧化物歧化酶（SOD）、细胞色素 P450（CYP）1A1 和谷胱甘肽-S-转移酶（GST）的 mRNA 表达水平在第 3 天显著诱导，之后恢复到初始水平。0.3 和 3μg/L BaP 还诱导了鳃和肝胰腺中的 7-乙氧基resorufin O-脱乙基酶（EROD）、GST 和 SOD 活性，而 BaP 暴露后 T-AOC 和 GSH 含量降低。研究还表明，0.3 和 3μg/L BaP 造成了损伤效应，而 0.03μg/L BaP 处理则没有表现出任何损伤效应。此外，根据相关分析结果，我们得出结论，肝胰腺中的 EROD 活性、GST 活性和 GSH 含量可作为防御生物标志物；鳃和肝胰腺中的 DNA 链断裂、脂质过氧化（LPO）水平和蛋白质羰基（PC）含量可作为损伤生物标志物；肝胰腺中的 EROD 活性、GST 活性、GSH 含量以及 DNA 链断裂、LPO 水平和 PC 含量的损伤效应可作为联合生物标志物。这些结果不仅提供了有关可有效应用于监测水生环境污染的潜在生物标志物的信息，还提供了有关该物种毒性作用的信息。
