Hospital General Dr. Manuel Gea González, Ciudad de México, Mexico; Hospital General de México, Ciudad de México, Mexico.
Braz J Infect Dis. 2012 Sep-Oct;16(5):432-5. doi: 10.1016/j.bjid.2012.08.012. Epub 2012 Sep 10.
An aeromycological study verifies the presence and quantifies the concentration of fungal propagules in the air. It is very important in the hospital setting because of the increasing numbers of immunosuppressed and severely ill patients. The objective of this study was to determine the concentration of fungi in the air of the intensive care unit (ICU) of "Dr. Manuel Gea González" General Hospital.
This is a descriptive, observational cross-sectional study. Air samples were obtained with a single stage Thermo-Andersen Viable Particle Sampler (Thermo Electron Corporation - Massachusetts, U.S.A.) in a Petri dish with potato dextrose agar for 15 minutes at two different times (morning and afternoon) and heights (1 and 1.5 meters). The Petri dishes were incubated for five to seven days at 27° C, the number of colonies was counted, and the total CFU/m(3) was determined. The isolated fungal genera were identified by morphological features. Epi Info v. 3.4.3 © was used for statistical analysis.
The mean concentration of fungi in the air of the ICU was 85.08 ± 29.19 CFU/m(3); while in the outside air it was 84.3 ± 17.23 CFU/m(3) (p = 0.96). The fungi isolated were: Cladosporium spp., Penicillium spp., Aspergillus spp. (non-fumigatus), Fusarium spp., Exophiala spp., Syncephalastrum spp., and Acremonium spp.
Fungal spores were found in the air of the ICU and Cladosporium spp. was the most frequently isolated fungi. There was no difference according to sampling time or height.
空气微生物学研究通过验证空气中真菌繁殖体的存在和量化其浓度，来检测空气的微生物情况。由于免疫抑制和重症患者的数量不断增加，该研究在医院环境中非常重要。本研究的目的是确定“曼努埃尔·赫亚·冈萨雷斯”综合医院重症监护病房（ICU）空气中真菌的浓度。
这是一项描述性、观察性的横断面研究。使用单级 Thermo-Andersen 可培养粒子采样器（美国马萨诸塞州 Thermo Electron 公司）在培养皿中收集 15 分钟的空气样本，培养皿中含有土豆葡萄糖琼脂，采样时间为两个不同的时间（上午和下午）和高度（1 米和 1.5 米）。将培养皿在 27°C 下孵育五到七天，计算菌落数量，并确定总 CFU/m³。通过形态特征鉴定分离的真菌属。使用 Epi Info v. 3.4.3 © 进行统计分析。
ICU 空气中真菌的平均浓度为 85.08 ± 29.19 CFU/m³；而在室外空气中的浓度为 84.3 ± 17.23 CFU/m³（p = 0.96）。分离出的真菌包括：枝孢属、青霉属、曲霉属（非烟曲霉）、镰刀菌属、外瓶霉属、束梗孢属和节菱孢属。
在 ICU 空气中发现了真菌孢子，最常分离到的真菌是枝孢属。采样时间或高度没有差异。
