Department of Dermatology and Institute of Hair and Cosmetic Medicine, Yonsei University, Wonju College of Medicine, Wonju, Republic of Korea.
Neuropeptides. 2013 Feb;47(1):51-7. doi: 10.1016/j.npep.2012.07.008. Epub 2012 Sep 10.
The hair follicle is a widely available and instructive miniature organ in the human body that experiences major histocompatibility complex (MHC) class I dependent immune privilege (IP). There are various regulation factors that act on the generation, maintenance, and collapse of hair follicle IP. Neuropeptides such as calcitonin gene-related peptide (CGRP) are created in many organs, including skin, and display various immune regulation effects. The purpose of this study was to investigate the phenotypic effect of CGRP on the hair follicle's IP. First, we used interferon-γ (IFN-γ) to generate ectopic MHC antigen expression model in cultured human hair follicles as previously described. Then, we examined the effects of CGRP on the regulation of ectopic MHC antigen expression in cultured human hair follicles using reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemical staining techniques. IFN-γ (75 IU/ml) induced ectopic MHC expression. CGRP down-regulated INF-γ-induced ectopic MHC class I mRNA expression. These down-regulated effects were especially evident in 10(-8)M. In addition, CGRP also suppressed the staining intensity related to the expression of MHC class I and MHC class I-pathway related molecules (β2-microglobulin), but had no effect on MHC class II antigen expression. Taken together, these results indicate that CGRP might be an important regulatory factor for IP maintenance and restoration of IP via suppression of MHC class I antigen.
毛囊是人体中一种广泛存在且具有启示性的微型器官，经历着主要组织相容性复合体（MHC）I 类依赖性免疫豁免（IP）。有各种调节因素作用于毛囊 IP 的产生、维持和崩溃。降钙素基因相关肽（CGRP）等神经肽在包括皮肤在内的许多器官中产生，并表现出各种免疫调节作用。本研究旨在探讨 CGRP 对毛囊 IP 的表型影响。首先，我们使用干扰素-γ（IFN-γ）按照先前的描述在培养的人毛囊中产生异位 MHC 抗原表达模型。然后，我们使用逆转录-聚合酶链反应（RT-PCR）和免疫组织化学染色技术检查 CGRP 对培养的人毛囊中异位 MHC 抗原表达的调节作用。IFN-γ（75IU/ml）诱导异位 MHC 表达。CGRP 下调 IFN-γ诱导的异位 MHC I 类 mRNA 表达。在 10(-8)M 时，这些下调作用尤为明显。此外，CGRP 还抑制了与 MHC I 类和 MHC I 类途径相关分子（β2-微球蛋白）表达相关的染色强度，但对 MHC II 类抗原表达没有影响。综上所述，这些结果表明，CGRP 可能是 IP 维持和恢复的重要调节因子，通过抑制 MHC I 类抗原来实现。
