Jin Xing-Hai, Pi Long-Quan, Lee Won-Soo
Department of Dermatology and Institute of Hair and Cosmetic Medicine, Yonsei University Wonju College of Medicine, Wonju, Korea.
Department of Dermatology, Affiliated Hospital of Yanbian University, Yanji, China.
Ann Dermatol. 2019 Oct;31(5):511-517. doi: 10.5021/ad.2019.31.5.511. Epub 2019 Aug 30.
Klotho protein plays a pivotal role in aging regulation. However, it is unclear whether klotho is expressed in human hair follicles and is correlated with hair growth.
The purpose of this study was to determine the expression pattern and role of klotho in human hair follicles.
We examined the klotho expression patterns in human hair follicles from young and aged donors. Furthermore, we examined the functional roles of klotho on human hair growth using klotho siRNA and klotho recombinant protein.
Interestingly, klotho was expressed in human hair follicles at both gene and protein levels. In hair follicles, prominent klotho expression was mainly observed in the outermost regions of the outer root sheath and hair bulb matrix cells. Quantification of klotho protein expression in young and aged donors showed that klotho expression decreased with aging. In human hair follicle organ culture, klotho silencing promoted premature catagen induction and inhibited human hair growth. Otherwise, klotho protein prolonged human hair growth.
These results indicate that klotho might be an important regulatory factor for human hair growth and hair cycle change.
klotho蛋白在衰老调节中起关键作用。然而，klotho是否在人毛囊中表达以及与头发生长是否相关尚不清楚。
本研究旨在确定klotho在人毛囊中的表达模式及作用。
我们检测了年轻和老年供体人毛囊中的klotho表达模式。此外，我们使用klotho小干扰RNA（siRNA）和klotho重组蛋白检测了klotho对人毛发生长的功能作用。
有趣的是，klotho在人毛囊中基因和蛋白水平均有表达。在毛囊中，klotho的显著表达主要见于外根鞘最外层区域和毛球基质细胞。对年轻和老年供体中klotho蛋白表达的定量分析表明，klotho表达随衰老而降低。在人毛囊器官培养中，klotho沉默促进了早衰期的提前诱导并抑制了人毛发生长。相反，klotho蛋白延长了人毛发生长。
这些结果表明，klotho可能是人毛发生长和毛发周期变化的重要调节因子。
