胆固醇与双分子层膜中的所有脂质相互作用。对模型的启示。
Cholesterol interacts with all of the lipid in bilayer membranes. Implications for models.
作者信息
Singer M A, Finegold L
机构信息
Department of Medicine, Queen's University, Kingston, Ontario, Canada.
出版信息
Biophys J. 1990 Jan;57(1):153-6. doi: 10.1016/S0006-3495(90)82516-9.
Abstract
The interaction of cholesterol with lipid membranes has been studied by differential scanning calorimetry on liposomes, a technique which involves only the natural lipids, with no exogeneous probes. The influence of cholesterol at different molar percent concentrations c on the enthalpy delta H of the main gel to liquid crystal phase transition of saturated phosphatidylcholines of acyl chain length n = 12-20 was well represented by delta H = -9.43 + 1.01n - 0.268c kcal/mol. The linear dependence of delta H simultaneously upon chain length n and upon cholesterol concentration c shows clearly that cholesterol interacts with the deeper part of the lipids, as well as the superficial parts. This observation is not accommodated in any of the current models of cholesterol-lipid interactions.
摘要
通过对脂质体进行差示扫描量热法研究了胆固醇与脂质膜的相互作用，该技术仅涉及天然脂质，不使用外源探针。在酰基链长度n = 12 - 20的饱和磷脂酰胆碱从主要凝胶相向液晶相转变的过程中，不同摩尔百分比浓度c的胆固醇对焓变ΔH的影响可以很好地用ΔH = -9.43 + 1.01n - 0.268c千卡/摩尔来表示。ΔH同时对链长度n和胆固醇浓度c的线性依赖性清楚地表明，胆固醇不仅与脂质的表面部分相互作用，还与脂质的更深部分相互作用。目前任何胆固醇-脂质相互作用模型都无法解释这一观察结果。
相似文献
1
Cholesterol interacts with all of the lipid in bilayer membranes. Implications for models.胆固醇与双分子层膜中的所有脂质相互作用。对模型的启示。
Biophys J. 1990 Jan;57(1):153-6. doi: 10.1016/S0006-3495(90)82516-9.
2
N-palmitoyl sphingomyelin bilayers: structure and interactions with cholesterol and dipalmitoylphosphatidylcholine.N-棕榈酰鞘磷脂双层膜：结构及其与胆固醇和二棕榈酰磷脂酰胆碱的相互作用
Biochemistry. 1996 Jun 18;35(24):8025-34. doi: 10.1021/bi9528356.
3
A DSC and FTIR spectroscopic study of the effects of the epimeric 4-cholesten-3-ols and 4-cholesten-3-one on the thermotropic phase behaviour and organization of dipalmitoylphosphatidylcholine bilayer membranes: comparison with their 5-cholesten analogues.DSC 和 FTIR 光谱研究差向异构体 4-胆甾烯-3-醇和 4-胆甾烯-3-酮对二棕榈酰磷脂酰胆碱双层膜热致相态行为和组织的影响：与 5-胆甾烯类似物的比较。
Chem Phys Lipids. 2014 Jan;177:71-90. doi: 10.1016/j.chemphyslip.2013.11.008. Epub 2013 Dec 1.
4
Cholesterol-multilipid interactions in bilayers.
Chem Phys Lipids. 1991 May-Jun;58(1-2):169-73. doi: 10.1016/0009-3084(91)90122-r.
5
Phosphatidylcholine-cholesterol interactions: bilayers of heteroacid lipids containing linoleate lose calorimetric transitions at low cholesterol concentration.磷脂酰胆碱 - 胆固醇相互作用：含有亚油酸的杂酸脂质双层在低胆固醇浓度下失去量热转变。
Biochim Biophys Acta. 1989 Jul 24;983(1):51-5. doi: 10.1016/0005-2736(89)90379-9.
6
Thermodynamics of transfer of cholesterol from gel to fluid phases of phospholipid bilayers.胆固醇从磷脂双层的凝胶相转移至流体相的热力学。
Biochim Biophys Acta. 1996 Mar 13;1279(2):190-6. doi: 10.1016/0005-2736(95)00267-7.
7
A calorimetric and spectroscopic comparison of the effects of lathosterol and cholesterol on the thermotropic phase behavior and organization of dipalmitoylphosphatidylcholine bilayer membranes.角鲨烯和胆固醇对二棕榈酰磷脂酰胆碱双层膜热致相行为和组织的影响的量热法和光谱学比较。
Biochemistry. 2011 Nov 22;50(46):9982-97. doi: 10.1021/bi200721j. Epub 2011 Oct 26.
8
Interaction of octyl-beta-thioglucopyranoside with lipid membranes.辛基-β-硫代葡萄糖苷与脂质膜的相互作用。
Biophys J. 1997 Nov;73(5):2565-74. doi: 10.1016/S0006-3495(97)78285-7.
9
Studies of the structure and organization of cationic lipid bilayer membranes: calorimetric, spectroscopic, and x-ray diffraction studies of linear saturated P-O-ethyl phosphatidylcholines.阳离子脂质双层膜的结构与组织研究：线性饱和P-O-乙基磷脂酰胆碱的量热法、光谱法和X射线衍射研究
Biophys J. 2001 Mar;80(3):1329-42. doi: 10.1016/S0006-3495(01)76106-1.
10
Cholesterol interactions with tetracosenoic acid phospholipids in model cell membranes: role of the double-bond position.
Biochemistry. 1990 Apr 10;29(14):3466-71. doi: 10.1021/bi00466a007.
引用本文的文献
1
In situ precipitation: a novel approach for preparation of iron-oxide magnetoliposomes.原位沉淀法：一种制备氧化铁磁性脂质体的新方法。
Int J Nanomedicine. 2014 May 23;9:2607-17. doi: 10.2147/IJN.S59859. eCollection 2014.
2
Effects of natural and enantiomeric cholesterol on the thermotropic phase behavior and structure of egg sphingomyelin bilayer membranes.天然胆固醇及其对映体对卵鞘磷脂双层膜热致相行为和结构的影响。
Biophys J. 2003 Feb;84(2 Pt 1):1038-46. doi: 10.1016/S0006-3495(03)74920-0.
3
Calorimetry of apolipoprotein-A1 binding to phosphatidylcholine-triolein-cholesterol emulsions.载脂蛋白A1与磷脂酰胆碱-三油酸甘油酯-胆固醇乳剂结合的量热法。
Biophys J. 1996 Jan;70(1):330-8. doi: 10.1016/S0006-3495(96)79574-7.
4
The effects of bilirubin on the thermal properties of phosphatidylcholine bilayers.胆红素对磷脂酰胆碱双层膜热性质的影响。
Biophys J. 1993 Jul;65(1):101-5. doi: 10.1016/S0006-3495(93)81047-6.
5
Comparative differential scanning calorimetric and FTIR and 31P-NMR spectroscopic studies of the effects of cholesterol and androstenol on the thermotropic phase behavior and organization of phosphatidylcholine bilayers.胆固醇和雄烯醇对磷脂酰胆碱双层膜热致相行为及结构影响的差示扫描量热法、傅里叶变换红外光谱法和31P核磁共振光谱法的比较研究
Biophys J. 1994 Mar;66(3 Pt 1):741-52. doi: 10.1016/s0006-3495(94)80850-1.
本文引用的文献
1
Thermal behavior of stearoylsphingomyelin-cholesterol dispersions.硬脂酰鞘磷脂 - 胆固醇分散体的热行为
Biochemistry. 1981 Dec 8;20(25):7115-8. doi: 10.1021/bi00528a010.
2
The metastability of saturated phosphatidylcholines depends on the acyl chain length.饱和磷脂酰胆碱的亚稳定性取决于酰基链的长度。
Biochim Biophys Acta. 1986 Mar 13;855(3):417-20. doi: 10.1016/0005-2736(86)90086-6.
3
A differential scanning calorimetric study of the thermotropic phase behavior of model membranes composed of phosphatidylcholines containing linear saturated fatty acyl chains.对由含有线性饱和脂肪酰链的磷脂酰胆碱组成的模型膜的热致相行为的差示扫描量热研究。
Biochemistry. 1987 Sep 22;26(19):6118-26. doi: 10.1021/bi00393a026.
4
Spin-label studies on phosphatidylcholine-cholesterol membranes: effects of alkyl chain length and unsaturation in the fluid phase.磷脂酰胆碱 - 胆固醇膜的自旋标记研究：液相中烷基链长度和不饱和度的影响
Biochim Biophys Acta. 1986 Jan 29;854(2):307-17. doi: 10.1016/0005-2736(86)90124-0.
5
Theory of thermal anomalies in the specific heat of lipid bilayers containing cholesterol.含胆固醇脂质双层比热中热异常的理论。
Biophys J. 1989 Oct;56(4):661-7. doi: 10.1016/S0006-3495(89)82713-4.
6
Lipid chains and cholesterol in model membranes: a Monte Carlo Study.模型膜中的脂质链与胆固醇：一项蒙特卡洛研究。
Biochemistry. 1989 May 2;28(9):3687-91. doi: 10.1021/bi00435a010.
7
Intrinsic molecules in lipid membranes change the lipid-domain interfacial area: cholesterol at domain interfaces.脂质膜中的内在分子会改变脂质域的界面面积：结构域界面处的胆固醇。
Biochim Biophys Acta. 1989 Feb 27;979(2):166-76. doi: 10.1016/0005-2736(89)90432-x.
8
Investigation of phase transitions of lipids and lipid mixtures by sensitivity differential scanning calorimetry.利用灵敏度差示扫描量热法研究脂质及脂质混合物的相变
Proc Natl Acad Sci U S A. 1976 Nov;73(11):3862-6. doi: 10.1073/pnas.73.11.3862.
9
High-sensitivity scanning calorimetric study of mixtures of cholesterol with dimyristoyl- and dipalmitoylphosphatidylcholines.胆固醇与二肉豆蔻酰磷脂酰胆碱和二棕榈酰磷脂酰胆碱混合物的高灵敏度扫描量热研究。
Biochemistry. 1978 Jun 13;17(12):2464-8. doi: 10.1021/bi00605a034.
Zotero 插件