利用灵敏度差示扫描量热法研究脂质及脂质混合物的相变
Investigation of phase transitions of lipids and lipid mixtures by sensitivity differential scanning calorimetry.
作者信息
Mabrey S, Sturtevant J M
出版信息
Proc Natl Acad Sci U S A. 1976 Nov;73(11):3862-6. doi: 10.1073/pnas.73.11.3862.
Abstract
High sensitivity differential scanning calorimetry is applied to the study of the thermotropic behavior of mixtures of synthetic phospholipids in multilamellar aqueous suspensions. The systems dimyristoylphosphatidylcholine dipalmitoylphosphatidylcholine, and dimyristoylphosphatidylethanolamine-distearoylphosphatidylcholine, although definitely nonideal, exhibit essentially complete miscibility in both gel and liquid crystalline states, while the system dilauroylphosphatidylcholine-distearoylphosphatidylcholine is monotectic with lateral phase separation in the gel state. Comparison of the observed transition curves with theoretical curves calculated from the calorimetrically determined phase diagrams supports a literal interpretation of the phase diagrams.
摘要
高灵敏度差示扫描量热法被用于研究多层水悬浮液中合成磷脂混合物的热致行为。二肉豆蔻酰磷脂酰胆碱-二棕榈酰磷脂酰胆碱体系以及二肉豆蔻酰磷脂酰乙醇胺-二硬脂酰磷脂酰胆碱体系，尽管肯定是非理想体系，但在凝胶态和液晶态下都表现出基本完全互溶，而二月桂酰磷脂酰胆碱-二硬脂酰磷脂酰胆碱体系是单变的，在凝胶态下存在横向相分离。将观察到的转变曲线与根据量热法测定的相图计算出的理论曲线进行比较，支持了对相图的字面解释。
相似文献
1
Investigation of phase transitions of lipids and lipid mixtures by sensitivity differential scanning calorimetry.利用灵敏度差示扫描量热法研究脂质及脂质混合物的相变
Proc Natl Acad Sci U S A. 1976 Nov;73(11):3862-6. doi: 10.1073/pnas.73.11.3862.
2
A differential scanning calorimetry study of the interaction of alpha-tocopherol with mixtures of phospholipids.α-生育酚与磷脂混合物相互作用的差示扫描量热法研究
Biochim Biophys Acta. 1987 Apr 9;898(2):214-22. doi: 10.1016/0005-2736(87)90040-x.
3
A calorimetry and deuterium NMR study of mixed model membranes of 1-palmitoyl-2-oleylphosphatidylcholine and saturated phosphatidylcholines.1-棕榈酰-2-油酰磷脂酰胆碱与饱和磷脂酰胆碱混合模型膜的量热法和氘核磁共振研究
Biochim Biophys Acta. 1985 Jul 25;817(2):261-70. doi: 10.1016/0005-2736(85)90027-6.
4
Thermotropic behavior of phosphatidylethanolamine-cholesterol and phosphatidylethanolamine-phosphatidylcholine-cholesterol mixtures.磷脂酰乙醇胺 - 胆固醇以及磷脂酰乙醇胺 - 磷脂酰胆碱 - 胆固醇混合物的热致行为
Biochemistry. 1980 Oct 14;19(21):4908-13. doi: 10.1021/bi00562a032.
5
Interconversion of bilayer phase transition temperatures between phosphatidylethanolamines and phosphatidylcholines.磷脂酰乙醇胺和磷脂酰胆碱之间双层相变温度的相互转换。
Biochim Biophys Acta. 1994 Jan 3;1189(1):7-12. doi: 10.1016/0005-2736(94)90273-9.
6
On the miscibility of cardiolipin with 1,2-diacyl phosphoglycerides: binary mixtures of dimyristoylphosphatidylethanolamine and tetramyristoylcardiolipin.关于心磷脂与1,2 - 二酰基磷酸甘油酯的混溶性：二肉豆蔻酰磷脂酰乙醇胺与四肉豆蔻酰心磷脂的二元混合物
Biochim Biophys Acta. 2011 Mar;1808(3):774-83. doi: 10.1016/j.bbamem.2010.12.010. Epub 2010 Dec 21.
7
Phospholipid miscibility in ternary mixtures.三元混合物中的磷脂混溶性。
Biochim Biophys Acta. 1982 May 21;688(1):23-8. doi: 10.1016/0005-2736(82)90574-0.
8
Induction of lateral phase separations in binary lipid mixtures by alcohol.醇诱导二元脂质混合物中的侧向相分离。
Biochemistry. 1987 Jan 13;26(1):46-51. doi: 10.1021/bi00375a007.
9
New approaches to the simulation of heat-capacity curves and phase diagrams of pseudobinary phospholipid mixtures.模拟伪二元磷脂混合物热容曲线和相图的新方法。
Biophys J. 1996 Dec;71(6):3215-28. doi: 10.1016/S0006-3495(96)79515-2.
10
The use of differential scanning calorimetry and differential thermal analysis in studies of model and biological membranes.差示扫描量热法和差热分析在模型膜和生物膜研究中的应用
Chem Phys Lipids. 1982 May;30(2-3):229-59. doi: 10.1016/0009-3084(82)90053-6.
引用本文的文献
1
Kinetics of light-induced mesophase transitions in azo-benzene amphiphiles containing lyotropic liquid crystals.含溶致液晶的偶氮苯两亲物中光致中间相转变的动力学
J Appl Crystallogr. 2025 Jul 8;58(Pt 4):1322-1331. doi: 10.1107/S1600576725004923. eCollection 2025 Aug 1.
2
Investigation of Cell Damage Induced by Silver Nanoparticles in a Model Cell System.银纳米颗粒在模型细胞系统中诱导的细胞损伤研究。
Pharmaceutics. 2025 Mar 21;17(4):398. doi: 10.3390/pharmaceutics17040398.
3
A Label-Free Multitechnique Approach to Characterize the Interaction of Bioactive Compounds with Biomimetic Interfaces.一种用于表征生物活性化合物与仿生界面相互作用的无标记多技术方法。
Small Sci. 2024 Mar 1;4(4):2300271. doi: 10.1002/smsc.202300271. eCollection 2024 Apr.
4
Dense, continuous membrane labeling and expansion microscopy visualization of ultrastructure in tissues.组织中超微结构的致密、连续膜标记和扩展显微镜可视化
Nat Commun. 2025 Feb 12;16(1):1579. doi: 10.1038/s41467-025-56641-z.
5
Modification of Liposomal Properties by an Engineered Gemini Surfactant.工程化 Gemini 表面活性剂对脂质体性质的修饰
Langmuir. 2025 Feb 11;41(5):3042-3052. doi: 10.1021/acs.langmuir.4c03043. Epub 2025 Jan 25.
6
5-PC as a Lipid Probe Molecule and as a Second Phospholipid in Binary Phospholipid Mixtures: Saturation Recovery EPR Studies.5-PC作为脂质探针分子以及二元磷脂混合物中的第二种磷脂：饱和恢复电子顺磁共振研究
Int J Mol Sci. 2024 Nov 30;25(23):12913. doi: 10.3390/ijms252312913.
7
Multifocal lipid membrane characterization by combination of DAS-deconvolution and anisotropy.通过DAS去卷积和各向异性相结合的方法对多焦点脂质膜进行表征。
Biophys J. 2024 Dec 3;123(23):4135-4146. doi: 10.1016/j.bpj.2024.11.005. Epub 2024 Nov 7.
8
Thermal Remodeling of Human HDL Particles Reveals Diverse Subspecies.热重塑人高密度脂蛋白颗粒揭示了不同的亚种。
J Am Soc Mass Spectrom. 2024 Aug 7;35(8):2002-2007. doi: 10.1021/jasms.4c00228. Epub 2024 Jul 25.
9
Structure and thermodynamics of supported lipid membranes on hydrophobic van der Waals surfaces.疏水性范德华表面上支撑脂质膜的结构与热力学
Soft Matter. 2024 Jul 24;20(29):5724-5732. doi: 10.1039/d4sm00365a.
10
Microfluidic Transfection System and Temperature Strongly Influence the Efficiency of Transient Transfection.微流控转染系统和温度对瞬时转染效率有很大影响。
ACS Omega. 2024 May 3;9(19):21637-21646. doi: 10.1021/acsomega.4c02590. eCollection 2024 May 14.
本文引用的文献
1
Dynamics of lipids in membranes: Heterogeneity and the role of cholesterol.膜中脂质的动力学：异质性与胆固醇的作用
FEBS Lett. 1972 Jul 1;23(3):285-297. doi: 10.1016/0014-5793(72)80300-4.
2
Molecular interactions in mixed lecithin systems.混合卵磷脂系统中的分子相互作用。
Biochim Biophys Acta. 1970 Jan 6;196(1):35-44. doi: 10.1016/0005-2736(70)90163-x.
3
Thermal analysis of lipids, proteins and biological membranes. A review and summary of some recent studies.脂质、蛋白质及生物膜的热分析：近期一些研究的综述与总结
Chem Phys Lipids. 1969 Dec;3(4):304-56. doi: 10.1016/0009-3084(69)90040-1.
4
The effect of the polar headgroup on the lipid-cholesterol interaction: a monolayer and differential scanning calorimetry study.极性头部基团对脂质 - 胆固醇相互作用的影响：一项单分子层和差示扫描量热法研究。
Biochim Biophys Acta. 1973 Apr 25;307(1):1-19. doi: 10.1016/0005-2736(73)90020-5.
5
Calorimetric studies of dilute aqueous suspensions of bilayers formed from synthetic L- -lecithins.对由合成L-卵磷脂形成的双层稀水悬浮液的量热研究。
J Biol Chem. 1972 Oct 10;247(19):6071-5.
6
Lateral phase separation in phospholipid membranes.磷脂膜中的侧向相分离。
Biochemistry. 1973 Jun 5;12(12):2351-60. doi: 10.1021/bi00736a026.
7
Biomembrane phase transitions. Studies of lipid-water systems using differential scanning calorimetry.生物膜相变。利用差示扫描量热法对脂质 - 水体系的研究。
J Biol Chem. 1974 Apr 25;249(8):2512-21.
8
Fluorescence studies of chlorophyll a incorporated into lipid mixtures, and the interpretation of "phase" diagrams.掺入脂质混合物中的叶绿素a的荧光研究及“相”图的解读。
Biochim Biophys Acta. 1975 Nov 17;413(1):11-23. doi: 10.1016/0005-2736(75)90054-1.
9
Action of pancreatic phospholipase A2 on phosphatidylcholine bilayers in different physical states.胰腺磷脂酶A2对不同物理状态下磷脂酰胆碱双层膜的作用。
Biochim Biophys Acta. 1975 Oct 6;406(2):169-77. doi: 10.1016/0005-2736(75)90001-2.
10
Calorimetric and freeze-etch study of the influence of Mg2+ on the thermotropic behaviour of phosphatidylglycerol.镁离子对磷脂酰甘油热致行为影响的量热法和冷冻蚀刻研究
Chem Phys Lipids. 1975 Feb;14(1):97-101. doi: 10.1016/0009-3084(75)90021-3.
Zotero 插件