Department "Electro-induced and adhesive properties", Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Sofia, Bulgaria.
Clin Hemorheol Microcirc. 2012;52(2-4):337-48. doi: 10.3233/CH-2012-1609.
Hemocompatibility of elastic multiblock copolymers PDC, based on poly(p-dioxanone) (PPDO)/poly(ε-caprolactone)-segments, capable of a shape-memory effect, and PDD, based on PPDO/poly((adipinate-alt-1,4-butanediol)-co-(adipinate-alt-ethylene glycol)-co-adipinate-alt-diethylene glycol)-segments, was studied in order to assess their suitability for an application aiming at blood vessels regeneration. The results were compared with polypropylene (PP) which is a widely used blood-contacting material for devices as blood oxygenators and dialysis tubes. Protein adsorption studies showed diverse blood plasma proteins in a relatively high amount on both elastic polymers compared to the poor amount of plasma proteins adsorbed on PP. Study of the coagulation system revealed high thrombin formation on PDC and no difference in plasma kallikrein activation between elastic multiblock copolymers and the reference PP. Activation of complement system was higher for PDC followed by PDD and lower for PP. However, platelet adhesion and activation were hardly suppressed on the multiblock copolymers compared to the PP surface, where the number of adhered platelets and the activation rate were significant. The present results reveal that the tested multiblock copolymers with improved elastic properties and shape-memory capability (PDC) show low thrombogenicity and are promising candidates for vascular tissue engineering.
弹性多嵌段共聚物 PDC（基于聚对二氧环己酮（PPDO）/聚（ε-己内酯）段，具有形状记忆效应）和 PDD（基于 PPDO/聚（对苯二甲酸丙二醇酯-alt-1,4-丁二醇）-co-（对苯二甲酸丙二醇酯-alt-乙二醇）-co-对苯二甲酸丙二醇酯-alt-二甘醇）段）的血液相容性研究，旨在评估其在血管再生应用中的适用性。结果与广泛用于血液氧合器和透析管等设备的血液接触材料聚丙烯（PP）进行了比较。蛋白质吸附研究表明，与吸附在 PP 上的少量血浆蛋白相比，两种弹性聚合物上吸附了大量不同的血浆蛋白。凝血系统研究表明，PDC 上血栓酶的形成较高，而弹性多嵌段共聚物与参比 PP 之间血浆激肽释放酶的激活没有差异。补体系统的激活在 PDC 之后是 PDD，而在 PP 上较低。然而，与 PP 表面相比，血小板在多嵌段共聚物上的黏附和激活几乎没有被抑制，PP 表面上的血小板黏附数量和激活率都显著。目前的结果表明，具有改善的弹性性能和形状记忆能力的测试多嵌段共聚物（PDC）显示出低血栓形成性，是血管组织工程的有前途的候选材料。
