Crystallization of membrane proteins in bicelles.

The structural biology of membrane proteins remains a challenging field, partly due to the difficulty in obtaining high-quality crystals. We developed the bicelle method as a tool to aid with the production of membrane protein crystals. Bicelles are bilayer discs that are formed by a mixture of a detergent and a lipid. They combine the ease of use of detergents with the benefits of a lipidic medium. Bicelles maintain membrane proteins in a bilayer milieu, which is more similar to their native environment than detergent micelles. At the same time, bicelles are liquid at certain temperatures and they can be integrated into standard crystallization techniques without the need for specialized equipment.