Wang Tao, Cao Jian, Du Zhao-jie, Zhang Ya-bo, Liu Yan-pu, Wang Lei, Lei De-lin
Department of Oral and Maxillofacial Surgery, School of Stomatology, Fourth Military Medical University, Xi'an, China.
J Craniofac Surg. 2012 Sep;23(5):1524-8. doi: 10.1097/SCS.0b013e31825daab2.
Sympathetic nerve system has been proved to have important regulative effects to bone mass. However, the role of sympathetic nerve system in distraction osteogenesis (DO) is unclear. Here we show that the sympathetic nerve system plays an important role in mandibular DO. Thirty male Sprague-Dawley rats were divided into 2 groups at random. Right-side mandibular DO was performed on the 15 rats in control group (group A). Bilateral transection of cervical sympathetic trunk and right-side mandibular DO were performed on the 15 rats in the experimental group (group B). After operation, quantitative general observations, micro-computed tomography bone morphology analysis, and hematoxylin-eosin staining osseous tissue on new osteotylus in distraction gap were performed at consolidation time of 1, 14, and 28 days. SPSS 12.0 software package was used for statistical analysis. At 1 and 14 days of consolidation time, there was more continuous bone formation in the experimental group than that of the control group as determined by gross observation. Bone formation parameters including bone mineral density, bone volume-total volume ratio, bone trabeculae number as determined by micro-CT, and histological study of the test group were significantly higher than those of the control group (P < 0.05). No significant difference was noted between the 2 groups on consolidation time of 28 days. Our study suggested that the sympathetic innervation loss could improve mandibular DO and new bone formation, and the sympathetic nerve system might negatively regulate the process of DO.
交感神经系统已被证明对骨量具有重要的调节作用。然而，交感神经系统在牵张成骨（DO）中的作用尚不清楚。在此我们表明，交感神经系统在下颌骨DO中起重要作用。将30只雄性Sprague-Dawley大鼠随机分为2组。对对照组（A组）的15只大鼠进行右侧下颌骨DO。对实验组（B组）的15只大鼠进行双侧颈交感干横断术及右侧下颌骨DO。术后，在1、14和28天的愈合期进行定量大体观察、显微计算机断层扫描骨形态分析以及对牵张间隙新骨痂处骨组织进行苏木精-伊红染色。使用SPSS 12.0软件包进行统计分析。在愈合期的第1天和第14天，通过大体观察确定实验组的连续骨形成比对照组更多。通过显微CT测定的包括骨密度、骨体积-总体积比、骨小梁数量在内的骨形成参数以及试验组的组织学研究均显著高于对照组（P<0.05）。在28天的愈合期，两组之间未观察到显著差异。我们的研究表明，交感神经支配丧失可改善下颌骨DO和新骨形成，并且交感神经系统可能对DO过程起负调节作用。
