Department of Biochemistry and Molecular Biology, State University of New York Upstate Medical University, Syracuse, New York 13210, USA.
J Biol Chem. 2012 Oct 26;287(44):37089-97. doi: 10.1074/jbc.M112.389023. Epub 2012 Sep 12.
RNase mitochondrial RNA processing (MRP) is an essential, evolutionarily conserved endoribonuclease composed of 10 different protein subunits and a single RNA. RNase MRP has established roles in multiple pathways including ribosome biogenesis, cell cycle regulation, and mitochondrial DNA replication. Although each of these functions is important to cell growth, additional functions may exist given the essential nature of the complex. To identify novel RNase MRP substrates, we utilized RNA immunoprecipitation and microarray chip analysis to identify RNA that physically associates with RNase MRP. We identified several new potential substrates for RNase MRP including a cell cycle-regulated transcript, CTS1; the yeast homolog of the mammalian p27(Kip1), SIC1; and the U2 RNA component of the spliceosome. In addition, we found RNase MRP to be involved in the regulation of the Ty1 transposon RNA. These results reinforce and broaden the role of RNase MRP in cell cycle regulation and help to identify new roles of this endoribonuclease.
线粒体 RNA 加工酶（MRP）是一种必需的、进化上保守的内切核糖核酸酶，由 10 种不同的蛋白亚基和 1 种 RNA 组成。MRP 在多个途径中发挥作用，包括核糖体生物发生、细胞周期调控和线粒体 DNA 复制。尽管这些功能对细胞生长都很重要，但鉴于该复合物的必需性质，可能还存在其他功能。为了鉴定新的 MRP 核糖核酸酶底物，我们利用 RNA 免疫沉淀和微阵列芯片分析来鉴定与 MRP 核糖核酸酶物理结合的 RNA。我们鉴定了几个新的潜在的 MRP 核糖核酸酶底物，包括细胞周期调控转录物 CTS1；哺乳动物 p27(Kip1)的酵母同源物 SIC1；以及剪接体的 U2 RNA 成分。此外，我们发现 MRP 核糖核酸酶参与调节 Ty1 转座子 RNA。这些结果强化并拓宽了 MRP 核糖核酸酶在细胞周期调控中的作用，并有助于确定这种内切核糖核酸酶的新作用。
