Yanaka Noriyuki, Kanda Mayumi, Toya Keigo, Suehiro Haruna, Kato Norihisa
Department of Bioresource Science and Technology, Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima 739-8528, Japan.
Exp Ther Med. 2011 May;2(3):419-424. doi: 10.3892/etm.2011.238. Epub 2011 Mar 21.
We previously demonstrated that vitamin B6 suppresses tumorigenesis in the colon of mice and exerts an anti-inflammatory effect through the inhibition of NF-κB activation. As these effects resemble the pharmacological properties of thiazolidinedione (TZD), a synthetic peroxisome proliferator-activated receptor-γ (PPARγ) ligand, this study was designed to examine the effect of vitamin B6 on the activation of PPARγ and adipogenesis in 3T3-L1 adipocyte cells. Pyridoxal 5'-phosphate (PLP), one of the vitamin B6 derivatives, was shown to promote adipogenesis in the 3T3-L1 adipocytes. In addition, PLP specifically induced mRNA expression of PPARγ target genes in the 3T3-L1 adipocytes and enhanced the lipid accumulation and adipocyte fatty acid-binding protein (aP2) mRNA expression in NIH3T3 cells stably expressing PPARγ. Furthermore, the administration of vitamin B6 increased the expression of aP2 mRNA in mouse adipose tissues. Collectively, these observations suggest a novel function of vitamin B6 as an activator for PPARγ, which may contribute to the anti-tumor and anti-inflammatory effects of vitamin B6.
我们之前证明，维生素B6可抑制小鼠结肠肿瘤发生，并通过抑制核因子κB（NF-κB）激活发挥抗炎作用。由于这些作用类似于噻唑烷二酮（TZD）的药理特性，TZD是一种合成的过氧化物酶体增殖物激活受体γ（PPARγ）配体，本研究旨在检测维生素B6对3T3-L1脂肪细胞中PPARγ激活及脂肪生成的影响。维生素B6衍生物之一的磷酸吡哆醛（PLP）可促进3T3-L1脂肪细胞的脂肪生成。此外，PLP可特异性诱导3T3-L1脂肪细胞中PPARγ靶基因的mRNA表达，并增强稳定表达PPARγ的NIH3T3细胞中的脂质积累及脂肪细胞脂肪酸结合蛋白（aP2）mRNA表达。此外，给予维生素B6可增加小鼠脂肪组织中aP2 mRNA的表达。总体而言，这些观察结果提示维生素B6作为PPARγ激活剂的新功能，这可能有助于维生素B6的抗肿瘤和抗炎作用。
