乳腺癌细胞系中雌激素受体亚型及变体的分析
Analysis of estrogen receptor isoforms and variants in breast cancer cell lines.
作者信息
Al-Bader Maie, Ford Christopher, Al-Ayadhy Bushra, Francis Issam
机构信息
Departments of Physiology.
出版信息
Exp Ther Med. 2011 May;2(3):537-544. doi: 10.3892/etm.2011.226. Epub 2011 Mar 10.
In the present study, the expression of estrogen receptor (ER)α and ERβ isoforms in ER-positive (MCF7, T-47D and ZR-75-1) and ER-negative (MDA-MB-231, SK-BR-3, MDA-MB-453 and HCC1954) breast cancer cell lines was investigated. ERα mRNA was expressed in ER-positive and some ER-negative cell lines. ERα Δ3, Δ5 and Δ7 spliced variants were present in MCF7 and T-47D cells; ERα Δ5 and Δ7 spliced variants were detected in ZR-75-1 cells. MDA-MB-231 and HCC1954 cells expressed ERα Δ5 and Δ7 spliced variants. The ERβ1 variant was expressed in all of the cell lines and the ERβ2 variant in all of the ER-positive and some ER-negative cell lines (MDA-MB-231, MDA-MB-453 and SK-BR-3). MCF7, ZR-75-1, MDA-MB-453, HCC1954 and T-47D cells expressed ERβ5. All cell lines expressed an ERα 66-kDa protein band, and some expressed the truncated 42-kDa variant. ERβ1 was detected in all of the cell lines in addition to a 38-44 kDa variant. The results indicate that breast cancer cell lines widely used in research and reported as being ER-negative express ERα and/or ERβ mRNA and protein.
在本研究中，我们调查了雌激素受体（ER）α和ERβ亚型在雌激素受体阳性（MCF7、T-47D和ZR-75-1）及雌激素受体阴性（MDA-MB-231、SK-BR-3、MDA-MB-453和HCC1954）乳腺癌细胞系中的表达情况。ERα mRNA在雌激素受体阳性和部分雌激素受体阴性细胞系中表达。ERα Δ3、Δ5和Δ7剪接变体存在于MCF7和T-47D细胞中；在ZR-75-1细胞中检测到ERα Δ5和Δ7剪接变体。MDA-MB-231和HCC1954细胞表达ERα Δ5和Δ7剪接变体。ERβ1变体在所有细胞系中均有表达，ERβ2变体在所有雌激素受体阳性和部分雌激素受体阴性细胞系（MDA-MB-231、MDA-MB-453和SK-BR-3）中表达。MCF7、ZR-75-1、MDA-MB-453、HCC1954和T-47D细胞表达ERβ5。所有细胞系均表达一条66 kDa的ERα蛋白条带，部分细胞系还表达截短的42 kDa变体。除了38 - 44 kDa变体，在所有细胞系中均检测到ERβ1。结果表明，广泛用于研究且报道为雌激素受体阴性的乳腺癌细胞系表达ERα和/或ERβ mRNA及蛋白。
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