Yoshida Hitomi, Okumura Naoko, Nishimura Yuri, Kitagishi Yasuko, Matsuda Satoru
Department of Environmental Health Science, Nara Women's University, Nara 630-8506, Japan.
Exp Ther Med. 2011 Jul;2(4):629-632. doi: 10.3892/etm.2011.246. Epub 2011 Apr 1.
In the present study, we aimed to determine the effects of herbs or spices on the expression of presenilin 1, a molecule involved in γ-secretase activity and the generation of amyloid-β peptide in Alzheimer's disease. Western blot analysis revealed that presenilin 1 protein expression was down-regulated by stimulation with turmeric or cinnamon extracts in vitro, while the effects on presenilin 1 gene expression examined by reverse transcriptase-polymerase chain reaction were unaltered. Our results showed that curcumin, a component of turmeric, induced the down-regulation of presenilin 1 protein in Jurkat and K562 cell lines.
在本研究中，我们旨在确定草药或香料对早老素1表达的影响，早老素1是一种参与γ-分泌酶活性及阿尔茨海默病中β淀粉样肽生成的分子。蛋白质免疫印迹分析显示，在体外，姜黄或肉桂提取物刺激可下调早老素1蛋白的表达，而通过逆转录聚合酶链反应检测，其对早老素1基因表达的影响未改变。我们的结果表明，姜黄的一种成分姜黄素可诱导Jurkat和K562细胞系中早老素1蛋白的下调。
