Biasco G, Paganelli G M, Miglioli M, Brillanti S, Di Febo G, Gizzi G, Ponz de Leon M, Campieri M, Barbara L
Istituto di Clinica Medica e Gastroenterologia, University of Bologna, Italy.
Cancer Res. 1990 Feb 15;50(4):1156-9.
Cell proliferation kinetics of 30 patients affected by extensive ulcerative colitis in remission have been studied with autoradiography of rectal biopsies incubated with tritiated thymidine. The results have been compared with those of 20 control subjects without evidence of colonic diseases, and of 16 patients with multiple nonfamilial colonic adenomas. The labeling index was similar in the three groups (P = NS). On the contrary, the labeling frequency (SEM) in the upper 40% of the crypt (phi h value) was 0.04 +/- 0.01 in controls, 0.16 +/- 0.02 in ulcerative colitis, and 0.10 +/- 0.01 in adenoma patients (P less than 0.001 ulcerative colitis versus controls, P less than 0.01 adenomas versus controls, P = NS ulcerative colitis versus adenomas). The distribution of phi h values in ulcerative colitis showed a bimodal trend with 22 patients having mean phi h values similar to adenoma patients (0.10 +/- 0.01) and 8 with higher values (0.30 +/- 0.02). No relationship was found between phi h values and duration of colitis, age of patients, or age at onset of symptoms. These data show that cell kinetics studies can detect patients at particularly high risk of colon cancer, and that additional factors should determine colon cancer risk level in ulcerative colitis.
采用氚标记胸腺嘧啶核苷孵育直肠活检组织的放射自显影术，对30例缓解期广泛性溃疡性结肠炎患者的细胞增殖动力学进行了研究。将结果与20例无结肠疾病证据的对照受试者以及16例多发性非家族性结肠腺瘤患者的结果进行了比较。三组的标记指数相似（P = 无显著性差异）。相反，隐窝上部40%的标记频率（标准误）在对照组中为0.04±0.01，在溃疡性结肠炎患者中为0.16±0.02，在腺瘤患者中为0.10±0.01（溃疡性结肠炎与对照组相比P<0.001，腺瘤与对照组相比P<0.01，溃疡性结肠炎与腺瘤相比P = 无显著性差异）。溃疡性结肠炎患者的phi h值分布呈双峰趋势，22例患者的平均phi h值与腺瘤患者相似（0.10±0.01），8例患者的值较高（0.30±0.02）。未发现phi h值与结肠炎病程、患者年龄或症状发作年龄之间存在关联。这些数据表明，细胞动力学研究可以检测出患结肠癌风险特别高的患者，并且其他因素应决定溃疡性结肠炎患者的结肠癌风险水平。
