Qian Wei-Qing, Yin Hong
Department of Traumatology, Nanjing Hospital of Traditional Chinese Medicine, Nanjing University of Traditional Chinese Medicine, Nanjing.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2012 Jun;32(6):822-6.
To study the effects of administration or local application of epimedium on the fracture healing in osteoporosis rats.
Eighty-two 4-month old clean female rats, 210-250 g, were randomly divided into the experimental group (n = 75) and the control group (n = 7). The bilateral ovaries were resected in the experimental group, while only little fat tissue around the ovary was resected in the control group. Ten weeks after operation the osteoporosis model was successfully established verified by bone densitometry and scanning electron microscopy (SEM). The femur fracture models were established in the rest 72 rats of the experimental group. They were randomly divided into 3 groups, 24 in each group, i.e., the calcium phosphate cement (CPC) group (Group A), the CPC-epimedium group (Group B), and the epimedium administration group (Group C). The serum alkaline phosphatase (ALP) levels of the 3 groups were determined 2, 4, 8, and 12 weeks after surgery. The vitodynamical test and observation of the histological section were performed.
The serum ALP levels increased to some extent in the 3 groups 2, 4, and 8 weeks after bone fracture surgery. But the increase was more obvious in Group B with statistical difference shown when compared with Group A and C (P < 0.05). The ALP level in Group B decreased to the normal range till the 12th week. The bone fracture had not completely healed in Group C and A. Their ALP levels decreased to some extent, but were still maintained to a comparatively higher level, showing statistical difference when compared with that of Group B (P < 0.05). These results were agreeable with the results of the histological observation. Better bone activity promoting results were shown in Group B. The vitodynamical test results of the femur of Group B were all higher than those of Group A and C at each time point (P < 0.05).
Local application of epimedium could accelerate the fracture healing in osteoporosis rats. It showed better effects when compared with oral administration at the same dose.
研究淫羊藿给药或局部应用对骨质疏松大鼠骨折愈合的影响。
选取82只4月龄、体重210 - 250 g的清洁级雌性大鼠，随机分为实验组（n = 75）和对照组（n = 7）。实验组切除双侧卵巢，对照组仅切除卵巢周围少量脂肪组织。术后10周，通过骨密度测定和扫描电子显微镜（SEM）验证成功建立骨质疏松模型。将实验组其余72只大鼠建立股骨骨折模型，随机分为3组，每组24只，即磷酸钙骨水泥（CPC）组（A组）、CPC - 淫羊藿组（B组）和淫羊藿给药组（C组）。术后2、4、8和12周测定3组血清碱性磷酸酶（ALP）水平，进行活力测试并观察组织切片。
骨折术后2、4和8周，3组血清ALP水平均有不同程度升高，但B组升高更明显，与A组和C组比较有统计学差异（P < 0.05）。B组ALP水平至第12周降至正常范围。C组和A组骨折未完全愈合，其ALP水平有一定程度下降，但仍维持在较高水平，与B组比较有统计学差异（P < 0.05）。这些结果与组织学观察结果一致。B组促进骨活性效果更佳。B组股骨各时间点活力测试结果均高于A组和C组（P < 0.05）。
淫羊藿局部应用可加速骨质疏松大鼠骨折愈合，与相同剂量口服给药相比效果更佳。
