He Xufeng, Shen Qiang
Department of Orthopedic Surgery, Shanghai First People's Hospital, Shanghai Jiao Tong University, 85 Wu Jin Road, Shanghai 200080, China.
BMC Complement Altern Med. 2014 Dec 15;14:493. doi: 10.1186/1472-6882-14-493.
Radix Salviae miltiorrhizae is a herb frequently used within traditional Chinese medicine for the treatment of cardiovascular- and trauma-related diseases. Danshen is the dried root of Salviae miltiorrhizae, from which the polyphenolic compound Salvianolic acid B (Sal B) can be obtained. Sal B is a key component of Danshen. The aim of this study was to determine the effect of Sal B on the healing of long bones following trauma in a rat tibia fracture model.
Tibia fractures were created in 20 male Sprague Dawley rats. The animals were divided into two groups: (1) experimental group (n = 10); and (2) control group (n = 10). Rats in the experimental group were intraperitoneally administered with Sal B (40 mg/kg/d) for 3 weeks, while rats in the control group received an identical volume of physiological saline solution, administered in the same way. X-ray photographs were taken of all animals at the time points. Rats were euthanized at weeks 1, 3, 8 and 12 post-fracture. Fracture calluses were measured and callus sections were obtained and stained using hematoxylin and eosin (HE) and the calcium cobalt method. HE stained sections were observed and evaluated according to different grades of bone remodeling. Sections stained using the calcium cobalt method were analyzed with an imagine analysis system.
Data showed that callus growth was significantly greater in the experimental group compared with the control group (P < 0.05). Furthermore, histological scores in the Sal B-treated group were statistically higher than in the saline treated group at weeks 1, 3 and 8 post-fracture (P < 0.05). Alkaline phosphatase (ALP) activity was enhanced in the experimental group at weeks 1 and 3 post-fracture (P < 0.05).
Our results suggest that Sal B may accelerate early-stage fracture healing. Increased activity of ALP may be one factor which promotes the healing process. This pilot study provides brief insight into the effect of Sal B in fracture healing. These findings will contribute to the development of more and enhanced treatment options for trauma fracture patients.
丹参是一种常用于中医治疗心血管疾病和创伤相关疾病的草药。丹参是丹参的干燥根，从中可获得多酚类化合物丹酚酸B（Sal B）。Sal B是丹参的关键成分。本研究的目的是在大鼠胫骨骨折模型中确定Sal B对创伤后长骨愈合的影响。
选取20只雄性Sprague Dawley大鼠制造胫骨骨折。将动物分为两组：（1）实验组（n = 10）；（2）对照组（n = 10）。实验组大鼠腹腔注射Sal B（40 mg/kg/d），持续3周，而对照组大鼠接受相同体积的生理盐水溶液，以相同方式给药。在各个时间点对所有动物进行X线摄影。在骨折后第1、3、8和12周对大鼠实施安乐死。测量骨折痂，并获取痂组织切片，用苏木精和伊红（HE）染色以及钙钴法染色。根据不同等级的骨重塑对HE染色切片进行观察和评估。使用图像分析系统对钙钴法染色的切片进行分析。
数据显示，与对照组相比，实验组的骨痂生长明显更大（P < 0.05）。此外，在骨折后第1、3和8周，Sal B治疗组的组织学评分在统计学上高于生理盐水治疗组（P < 0.05）。在骨折后第1和3周，实验组的碱性磷酸酶（ALP）活性增强（P < 0.05）。
我们的结果表明，Sal B可能加速骨折早期愈合。ALP活性增加可能是促进愈合过程的一个因素。这项初步研究简要揭示了Sal B在骨折愈合中的作用。这些发现将有助于为创伤骨折患者开发更多更好的治疗方案。
