Deng Li-li, Zhong Ling, Lei Jian-rong, Tang Lin, Liu Ling, Xie Shu-qin, Liao Xiao-hui
Department of Internal Medicine, Chongqing Changan Hospital, Chongqing, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2012 Sep;28(9):907-10.
To investigate the protective effect of lipoxin A4 (LXA(4);) against rhabdomyolysis-induced acute kidney injury (AKI) and the possible mechanism.
The animal model of AKI was produced by intramuscular injection with glycerol. SD rats were randomly divided into 3 groups: the normal, AKI and LXA(4); groups. Blood urea nitrogen and serum creatinine levels were evaluated using a HITACHI 747 automatic analyzer. For histological examination, sections were stained with HE. The activity of myeloperoxidase (MPO) was detected by spectrophotometer. Expressions of TNF-α, IL-6 were determined by ELISA, and NF-κB in renal tissues was detected by Western blot analysis.
The levels of blood urea nitrogen and serum creatinine decreased, and the injury of kidney was improved significantly in rats treated with LXA(4); as compared with AKI rats (P<0.05). The levels of TNF-α and IL-6, the activity of MPO and the expression of NF-κB were significantly lower in LXA(4); group than in AKI group (P<0.05).
LXA(4); can significantly protect rats against AKI partly by blocking NF-κB activation and reducing inflammatory cytokine expression and inflammatory cells infiltration in the kidney.
探讨脂氧素A4（LXA4）对横纹肌溶解诱导的急性肾损伤（AKI）的保护作用及其可能机制。
通过肌肉注射甘油制备AKI动物模型。将SD大鼠随机分为3组：正常组、AKI组和LXA4组。使用日立747自动分析仪评估血尿素氮和血清肌酐水平。进行组织学检查时，切片用HE染色。用分光光度计检测髓过氧化物酶（MPO）的活性。通过ELISA测定TNF-α、IL-6的表达，通过蛋白质印迹分析检测肾组织中的NF-κB。
与AKI大鼠相比，LXA4治疗的大鼠血尿素氮和血清肌酐水平降低，肾损伤明显改善（P<0.05）。LXA4组TNF-α和IL-6水平、MPO活性及NF-κB表达均显著低于AKI组（P<0.05）。
LXA4可通过阻断NF-κB激活、减少炎性细胞因子表达及肾内炎性细胞浸润，显著保护大鼠免受AKI损伤。
