Sun Xue-lin, Bu Pei-li, Liu Jun-ni, Wang Xi, Wu Xin-ning, Zhao Li-xiang
Department of Cardiology, Shandong University, Jinan, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2012 Sep;28(9):972-4.
To observe the expression of mammalian sirtuin 1 (SIRT1) in right auricle tissues in patients presenting with atrial fibrillation (AF) and make clear the relationship between SIRT1 expression and oxidative stress.
A total of 38 patients with rheumatic heart disease were divided into 2 groups: AF group (AF lasted more than 6 months, n=25) and SR (sinus rhythm) group (n=13). The expression of SIRT1 in right auricle tissues harvested during heart operations was detected by immunohistochemistry. Oxidative stress was estimated by the amounts of malondialdehyde (MDA) and metallothionein (MT) and the activity of superoxide dismutase (SOD) which were detected using thiobarbituric acid reaction (TBA), enzyme-linked immunosorbent assay (ELISA) and xanthine oxidase assay, respectively.
Compared with the SR group, the expression of SIRT1 protein in the atrium significantly increased in AF group [P<0.05, (45.8±4.03)% vs (19.7±2.54)%]. In AF group, MDA was (7.24±1.05) nmol/mg, SOD (1034.25±84.32) U/mg and MT (7.21±1.46) μg/g, all being significantly higher than those in SR group[P<0.05, MDA: (3.01±0.47) nmol/mg; SOD: (723.63±65.23) U/mg; MT: (4.31±1.23) μg/g]. Spearman correlation indicated that the expression of SIRT1 had a significantly negative correlation with the amounts of MDA and MT and the activity of SOD (P<0.05, r=-0.447, -0.521, -0.394, respectively).
The expression of SIRT1 increased in patients with AF. SIRT1 maybe effects the AF by means of inhibiting the process of oxidative stress.
观察哺乳动物沉默信息调节因子1（SIRT1）在心房颤动（AF）患者右心耳组织中的表达情况，并明确SIRT1表达与氧化应激之间的关系。
选取38例风湿性心脏病患者，分为2组：房颤组（房颤持续时间超过6个月，n = 25）和窦性心律（SR）组（n = 13）。采用免疫组织化学法检测心脏手术中获取的右心耳组织中SIRT1的表达。分别采用硫代巴比妥酸反应（TBA）、酶联免疫吸附测定（ELISA）和黄嘌呤氧化酶法检测丙二醛（MDA）、金属硫蛋白（MT）的含量及超氧化物歧化酶（SOD）的活性，以评估氧化应激情况。
与SR组相比，房颤组心房中SIRT1蛋白表达显著增加[P < 0.05，（45.8±4.03）%对（19.7±2.54）%]。房颤组中，MDA为（7.24±1.05）nmol/mg，SOD为（1034.25±84.32）U/mg，MT为（7.21±1.46）μg/g，均显著高于SR组[P < 0.05，MDA：（3.01±0.47）nmol/mg；SOD：（723.63±65.23）U/mg；MT：（4.31±1.23）μg/g]。Spearman相关性分析表明，SIRT1的表达与MDA、MT的含量及SOD的活性呈显著负相关（P < 0.05，r分别为 - 0.447、 - 0.521、 - 0.394）。
房颤患者中SIRT1表达增加。SIRT1可能通过抑制氧化应激过程来影响房颤。
