Department of Pathology and Moores Cancer Center, University of California San Diego, La Jolla, CA 92093-0612, United States.
Curr Opin Cell Biol. 2012 Oct;24(5):662-9. doi: 10.1016/j.ceb.2012.08.005. Epub 2012 Sep 11.
Metastatic cancer cells produce invasive membrane protrusions called invadopodia and pseudopodia, which play a central role in driving cancer cell dissemination in the body. Malignant cells use these structures to attach to and degrade extracellular matrix proteins, generate force for cell locomotion, and to penetrate the vasculature. Recent work using unique subcellular fractionation methodologies combined with spatial genomic, proteomic, and phosphoproteomic profiling has provided insight into the invadopodiome and pseudopodiome signaling networks that control the protrusion of invasive membranes. Here I highlight how these powerful spatial 'omics' approaches reveal important signatures of metastatic cancer cells and possible new therapeutic targets aimed at treating metastatic disease.
转移性癌细胞产生侵袭性的膜突起，称为侵袭伪足和伪足，在驱动癌细胞在体内扩散中发挥核心作用。恶性细胞利用这些结构附着并降解细胞外基质蛋白，产生细胞运动的力，并穿透血管。最近使用独特的亚细胞分级分离方法结合空间基因组、蛋白质组和磷酸蛋白质组谱分析的工作，为控制侵袭性膜突起的侵袭伪足和伪足信号网络提供了深入了解。在这里，我将重点介绍这些强大的空间“组学”方法如何揭示转移性癌细胞的重要特征，以及可能针对治疗转移性疾病的新治疗靶点。
