School of Resources & Environment, Northeast Agricultural University, Harbin 150030, PR China.
J Hazard Mater. 2012 Oct 30;237-238:376-81. doi: 10.1016/j.jhazmat.2012.08.047. Epub 2012 Aug 31.
A stable four-member bacterial consortium, DNC5 that was capable of metabolizing atrazine was isolated from corn-planted soil. The main objective of this paper is to characterize the individual metabolic characteristics and the mutualism of the cultivable members in the consortium DNC5. Substrates utilizing character of each community member indicate that the primary organism in this consortium is Arthrobacter sp. DNS10, which was the only strain capable of mineralizing atrazine to cyanuric acid. Two secondary strains (Bacillus subtilis DNS4 and Variovorax sp. DNS12) utilized cyanuric acid during the atrazine degradation process. Meanwhile, we found that a metabolite (isopropylamine) inhibited the atrazine degrading species Arthrobacter sp. DNS10. The last strain (Arthrobacter sp. DNS9) of this consortium played a role in reducing this inhibition by utilizing isopropylamine for its growth. Altogether this is a new combination of isolates in an atrazine degrading consortium. The growth and the degradation rate of consortium DNC5 were faster than that of the single strain DNS10. The high degradation ability of the consortium showed good potential for atrazine biodegradation. This study will contribute toward a better understanding about metabolic activities of atrazine degrading consortium, which are generally considered to be responsible for atrazine mineralization in the natural environment.
从种植玉米的土壤中分离到能够代谢莠去津的稳定的四元细菌联合体 DNC5。本文的主要目的是表征联合体 DNC5 中可培养成员的个体代谢特征和共生关系。每个群落成员的底物利用特征表明，该联合体中的主要生物是节杆菌 sp. DNS10，它是唯一能够将莠去津矿化为三聚氰胺的菌株。两种次要菌株（枯草芽孢杆菌 DNS4 和鞘氨醇单胞菌 sp. DNS12）在莠去津降解过程中利用三聚氰胺。同时，我们发现一种代谢物（异丙胺）抑制了莠去津降解种节杆菌 sp. DNS10。该联合体的最后一个菌株（节杆菌 sp. DNS9）通过利用异丙胺来生长，从而减轻这种抑制作用。总的来说，这是一个在莠去津降解联合体中分离出的新组合。联合体 DNC5 的生长和降解速率均高于单一菌株 DNS10。该联合体具有较高的降解能力，显示出在莠去津生物降解方面的良好潜力。本研究将有助于更好地了解莠去津降解联合体的代谢活性，这些活性通常被认为是导致自然环境中莠去津矿化的原因。
