Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Mor. 62250, Mexico.
Toxicon. 2012 Dec 1;60(7):1223-7. doi: 10.1016/j.toxicon.2012.08.018. Epub 2012 Sep 5.
Hyaluronidases (Hyal) present in the venom of poisonous animals have been considered as "spreading factors" that facilitate a fast penetration of the venom in the prey. We have found that hyaluronidase from the tarantula Brachypelma vagans venom (BvHyal) displays a substrate-specific Hyal activity against hyaluronan. By using a combined strategy based on peptide sequencing and RT-PCR, we have cloned a BvHyal cDNA. Active recombinant BvHyal was efficiently expressed in a baculovirus system in insect cell.
在毒蛇的毒液中存在的透明质酸酶（Hyal）被认为是“扩散因子”，可以促进毒液快速渗透到猎物中。我们发现，来自狼蛛 Brachypelma vagans 毒液的透明质酸酶（BvHyal）对透明质酸显示出底物特异性的 Hyal 活性。通过使用基于肽测序和 RT-PCR 的组合策略，我们克隆了 BvHyal cDNA。活性重组 BvHyal 在杆状病毒系统中昆虫细胞中高效表达。
