College of Life Science, South China Normal University, Guangzhou 510631, China.
Neurosci Lett. 2012 Oct 24;528(2):201-4. doi: 10.1016/j.neulet.2012.08.056. Epub 2012 Sep 5.
Using a combination of electrophysiological recordings, behavioral tests and local pharmacological administration in hippocampus, we investigated in the present study the effects of nitric oxide (NO) synthase inhibitor N-nitro-l-arginine methyl ester (l-NAME) on the behavioral long-term potentiation (LTP) and maze learning performance in freely moving rats. The results showed as follows: (1) intrahippocampal l-NAME administration led to a defect in maze learning performance of the animals; (2) l-NAME treatment also substantially impaired the induction of the behavioral LTP in perforant pathway to dentate gyrus (PP-DG) pathway induced by maze learning task, while it had no significant effects on basic synaptic transmission in PP-DG pathway; Collectively, these results indicate that NO synthesis may be critical for the behavioral LTP in PP-DG pathway and maze learning performance.
在本研究中，我们使用电生理记录、行为测试和海马局部药物给药的组合，研究了一氧化氮 (NO) 合酶抑制剂 N-硝基-L-精氨酸甲酯 (l-NAME) 对自由移动大鼠行为长时程增强 (LTP) 和迷宫学习表现的影响。结果表明：（1）海马内给予 l-NAME 导致动物在迷宫学习表现上出现缺陷；（2）l-NAME 处理还显著损害了迷宫学习任务诱导的穿通纤维到齿状回 (PP-DG) 通路的行为 LTP 的诱导，而对 PP-DG 通路中的基本突触传递没有显著影响；总的来说，这些结果表明，NO 合成可能对 PP-DG 通路和迷宫学习表现中的行为 LTP 至关重要。
