Laboratory of Clinical Virology, Department of Microbiology and Immunology, Rega Institute for Medical Research, Minderbroedersstraat 10, BE-3000 Leuven, Belgium.
Infect Genet Evol. 2013 Jan;13:27-35. doi: 10.1016/j.meegid.2012.08.019. Epub 2012 Sep 12.
During the 2008-2009 rotavirus season, 10 G3P[6] rotavirus strains were isolated for the first time in Belgium, while an outbreak of G2P[6] strains occurred in the USA in 2005-2006. Partial sequencing of the 11 genome segments of the 10 Belgian G3P[6] strains revealed a clonal origin. Two of these strains, and a G2P[6] strain representative of the American outbreak, were selected and sequenced completely to analyze their evolutionary relationships. Genetic analysis revealed that all strains possessed a DS-1-like genotype constellation. The 2 Belgian G3P[6] strains showed >99% sequence identity at the nucleotide level and the American G2P[6] strain was phylogenetically closely related to the Belgian P[6] strains. These data suggest that reassortment(s) involving VP7 occurred recently, and that the prevalence of DS-1-like P[6] rotavirus strains need to be closely monitored because the currently licensed RVA vaccines contain neither the P[6] genotype nor strains with a complete human DS-1 genotype constellation.
在 2008-2009 年轮状病毒季节期间，比利时首次分离到 10 株 G3P[6]轮状病毒株，而 2005-2006 年美国发生了 G2P[6] 株的暴发。对 10 株比利时 G3P[6]株的 11 个基因组片段的部分测序揭示了它们的克隆起源。选择了其中的两个菌株以及代表美国暴发的 G2P[6] 株进行完全测序，以分析它们的进化关系。遗传分析显示，所有株均具有 DS-1 样基因型组合。这 2 株比利时 G3P[6]株在核苷酸水平上具有>99%的序列同一性，而美国 G2P[6]株与比利时 P[6]株在系统进化上密切相关。这些数据表明，最近发生了涉及 VP7 的重组，需要密切监测 DS-1 样 P[6]轮状病毒株的流行情况，因为目前许可的 RVA 疫苗既不包含 P[6]基因型，也不包含具有完整人类 DS-1 基因型组合的菌株。
