Ianiro Giovanni, Delogu Roberto, Fiore Lucia, Ruggeri Franco M
Dept. of Veterinary Public Health and Food Safety, Istituto Superiore di Sanità, Rome, Italy.
National Center for Immunobiologicals Research and Evaluation, Istituto Superiore di Sanità, Rome, Italy.
Infect Genet Evol. 2015 Jul;33:143-9. doi: 10.1016/j.meegid.2015.04.022. Epub 2015 Apr 23.
Group A rotaviruses (RVA) are the leading cause of acute gastroenteritis in young children, causing up to 450,000 deaths worldwide, mostly in developing countries. Most of RVA human infections in developed countries are related to five major G/P combinations: G1P[8], G2P[4], G3P[8], G4P[8] and G9P[8]. During the surveillance activity of RotaNet-Italy, three uncommon G3P[6] RVA strains, designated as RVA/Human-wt/ITA/NA01/2009/G3P[6], RVA/Human-wt/ITA/NA06/2009/G3P[6], and RVA/Human-wt/ITA/NA19/2009/G3P[6], were identified in the stools of children with diarrhea hospitalized in Southern Italy in 2009. Samples NA01, NA06 and NA19 were characterized as genotype G3P[6]. To investigate the three strains further, partial sequencing of the eleven genomic segments was performed. RVA strains NA01, NA06 and NA19 were found to share the rare genotype constellation: G3-P[6]-I2-R2-C2-M2-A2-N2-T2-E2-H2, which had not been reported previously in continental Italy. The phylogenetic analysis of the eleven genomic segments showed no evidence of zoonosis or inter-species reassortment at the origin of the Italian G3P[6] strains, indicating that they possessed DS-1-like genomic constellations similar to those detected previously in human cases in Africa and Europe. The analysis of the hypervariable regions of VP7 and VP4 (VP8*) revealed high amino acid identity between the Italian G3P[6] RVA strains involved in this study.
A组轮状病毒（RVA）是幼儿急性胃肠炎的主要病因，在全球范围内导致多达45万例死亡，其中大多数发生在发展中国家。在发达国家，大多数人类RVA感染与五种主要的G/P组合有关：G1P[8]、G2P[4]、G3P[8]、G4P[8]和G9P[8]。在意大利RotaNet监测活动期间，2009年在意大利南部住院腹泻儿童的粪便中鉴定出三株罕见的G3P[6] RVA毒株，分别命名为RVA/人类-wt/意大利/NA01/2009/G3P[6]、RVA/人类-wt/意大利/NA06/2009/G3P[6]和RVA/人类-wt/意大利/NA19/2009/G3P[6]。样本NA01、NA06和NA19被鉴定为G3P[6]基因型。为了进一步研究这三株毒株，对11个基因组片段进行了部分测序。发现RVA毒株NA01、NA06和NA19具有罕见的基因型组合：G3-P[6]-I2-R2-C2-M2-A2-N2-T2-E2-H2，此前在意大利大陆尚未有过报道。对11个基因组片段的系统发育分析表明，意大利G3P[6]毒株起源时没有人畜共患病或种间重配的迹象，这表明它们具有类似于之前在非洲和欧洲人类病例中检测到的DS-1样基因组组合。对VP7和VP4（VP8*）高变区的分析显示，本研究中涉及的意大利G3P[6] RVA毒株之间具有高度的氨基酸同一性。
