Disciplina de Genética, Departamento de Morfologia e Genética, Universidade Federal de São Paulo, São Paulo 04023-900, SP, Brazil.
Food Chem Toxicol. 2012 Dec;50(12):4412-20. doi: 10.1016/j.fct.2012.09.002. Epub 2012 Sep 12.
Canova activates macrophages and indirectly induces lymphocyte proliferation. Here we evaluated the effects of Canova in cyclophosphamide-treated non-human primates.
Twelve Cebus apella were evaluated. Four animals were treated with Canova only. Eight animals were treated with two doses of cyclophosphamide (50 mg/kg) and four of these animals received Canova. Body weight, biochemistry and hematologic analyses were performed for 40 days. Micronucleus and comet assays were performed for the evaluation of DNA damage.
We observed that cyclophosphamide induced abnormal WBC count in all animals. However, the group treated with cyclophosphamide plus Canova presented a higher leukocyte count than that which received only cyclophosphamide. Cyclophosphamide induced micronucleus and DNA damage in all animals. The frequency of these alterations was significantly lower in the Canova group than in the group without this medicine.
Our results demonstrated that Canova treatment minimizes cyclophosphamide myelotoxicity in C. apella.
Canova 可激活巨噬细胞并间接诱导淋巴细胞增殖。在此，我们评估了 Canova 在环磷酰胺处理的非人灵长类动物中的作用。
共评估了 12 只卷尾猴。4 只动物仅接受 Canova 治疗。8 只动物接受了两次环磷酰胺（50mg/kg）治疗，其中 4 只接受了 Canova 治疗。进行了 40 天的体重、生化和血液学分析。进行微核和彗星试验评估 DNA 损伤。
我们观察到环磷酰胺诱导了所有动物白细胞计数异常。然而，接受环磷酰胺加 Canova 治疗的组的白细胞计数高于仅接受环磷酰胺的组。环磷酰胺诱导了所有动物的微核和 DNA 损伤。在接受 Canova 治疗的组中，这些改变的频率明显低于未接受该药物治疗的组。
我们的结果表明，Canova 治疗可最大程度地减轻 C. apella 中环磷酰胺的骨髓毒性。
