Coelho Moreira Carlos Onete, de Fátima Ferreira Borges da Costa Joana, Leal Mariana Ferreira, Ferreira de Andrade Edilson, Rezende Alexandre Pingarilho, Imbeloni Aline Amaral, Pereira Carneiro Muniz José Augusto, de Arruda Cardoso Smith Marília, Burbano Rommel Rodríguez, de Assumpção Paulo Pimentel
Laboratório de Citogenética Humana, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brazil.
Homeopathy. 2012 Jan;101(1):74-9. doi: 10.1016/j.homp.2011.09.001.
Canova is a complex homeopathic medicine that enhances a specific immunologic responses against several exogenous and endogenous conditions. Canova activates macrophages both in vivo and in vitro.
We evaluated the effects of macrophages activated by Canova in vivo and ex vitro in the proliferation of lymphocytes. Canova was used to activate Cebus apella macrophages in vivo or ex vitro with Canova. Lymphocytes were cultured with the macrophage culture medium. The analysis of Canova effects in cultured lymphocytes was performed according to the cell cycle phase using flow cytometry. The Interferon gamma and Interleukin-5 cytokines quantification in these lymphocyte culture media was performed by Enzyme-linked immunosorbent assay (ELISA).
We observed that Canova actives macrophages in vivo and ex vitro. The lymphocytes cultured in a supplemented medium with macrophages activated by Canova treatment presented a higher number of proliferation cells than lymphocytes not exposed to macrophages activated by Canova. The Interferon gamma and Interleukin-5 cytokines were only observed in the medium of lymphocytes exposed to macrophages activated by Canova. Thus, Canova has potential as a new adjuvant therapy.
卡诺瓦是一种复杂的顺势疗法药物，可增强针对多种外源性和内源性病症的特定免疫反应。卡诺瓦在体内和体外均可激活巨噬细胞。
我们评估了卡诺瓦在体内和体外激活的巨噬细胞对淋巴细胞增殖的影响。使用卡诺瓦在体内或体外激活豚尾猴巨噬细胞。将淋巴细胞与巨噬细胞培养基一起培养。使用流式细胞术根据细胞周期阶段对培养的淋巴细胞中卡诺瓦的作用进行分析。通过酶联免疫吸附测定（ELISA）对这些淋巴细胞培养基中的干扰素γ和白细胞介素-5细胞因子进行定量。
我们观察到卡诺瓦在体内和体外均可激活巨噬细胞。在添加了经卡诺瓦处理激活的巨噬细胞的培养基中培养的淋巴细胞，其增殖细胞数量高于未接触经卡诺瓦激活的巨噬细胞的淋巴细胞。仅在接触经卡诺瓦激活的巨噬细胞的淋巴细胞培养基中观察到干扰素γ和白细胞介素-5细胞因子。因此，卡诺瓦有潜力作为一种新的辅助疗法。
