印度尼西亚野生褐家鼠中戊型肝炎病毒变异株的频繁检测和特征分析。
Frequent detection and characterization of hepatitis E virus variants in wild rats (Rattus rattus) in Indonesia.
机构信息
Immunobiology Laboratory, Faculty of Medicine, University of Mataram, Mataram, Indonesia.
出版信息
Arch Virol. 2013 Jan;158(1):87-96. doi: 10.1007/s00705-012-1462-0. Epub 2012 Sep 15.
One hundred sixteen rats (Rattus rattus) captured in Indonesia from 2011 to 2012 were investigated for the prevalence of hepatitis E virus (HEV)-specific antibodies and HEV RNA. Using an ELISA based on HEV genotype 4 with an ad hoc cutoff value of 0.500, 18.1 % of the rats tested positive for anti-HEV IgG. By nested RT-PCR, 14.7 % of the rats had rat HEV RNA, and none were positive for HEV genotype 1-4. A high HEV prevalence among rats was associated with lower sanitary conditions in areas with a high population density. Sixteen of the 17 HEV isolates obtained from infected rats showed >93.0 % nucleotide sequence identity within the 840-nucleotide ORF1-ORF2 sequence and were most closely related to a Vietnamese strain (85.9-87.9 % identity), while the remaining isolate differed from known rat HEV strains by 18.8-23.3 % and may belong to a novel lineage of rat HEV. These results suggest a wide distribution of rat HEV with divergent genomes.
从 2011 年到 2012 年，在印度尼西亚捕获了 116 只大鼠（Rattus rattus），对其进行了戊型肝炎病毒（HEV）特异性抗体和 HEV RNA 的流行情况调查。使用基于 HEV 基因型 4 的 ELISA，采用专门设定的 0.500 截断值，18.1%的大鼠检测出抗 HEV IgG 阳性。通过巢式 RT-PCR，14.7%的大鼠有大鼠 HEV RNA，但均未检测到 HEV 基因型 1-4。大鼠中存在较高的 HEV 流行率与人口密度较高地区较低的卫生条件有关。从感染大鼠中获得的 17 个 HEV 分离株中的 16 个在 840 个核苷酸 ORF1-ORF2 序列内显示出 >93.0%的核苷酸序列同一性，与越南株最为密切相关（85.9-87.9%的同一性），而其余分离株与已知的大鼠 HEV 株的差异为 18.8-23.3%，可能属于大鼠 HEV 的一个新谱系。这些结果表明大鼠 HEV 具有广泛的分布和不同的基因组。
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