Department of Biochemistry and Biotechnology, University of Thessaly, 26, Ploutonos & Aeolou str., Larissa 41221, Greece.
Arch Virol. 2013 Jan;158(1):103-11. doi: 10.1007/s00705-012-1472-y. Epub 2012 Sep 16.
Enteroviruses, the main cause of aseptic meningitis, consist of 100 serotypes, and many of them have been associated with large outbreaks. In the present study, a comparison of RFLP analysis of the 5' untranslated region (5'UTR) and sequencing of both the 5'UTR and VP1 regions was conducted for epidemiological linkage of 27 clinical enterovirus strains. The clinical enterovirus strains were clustered into five restriction profile groups. Even though the restriction profile clusters of clinical isolates were not related to those of the respective prototype strains, epidemiological relationships between the members of each cluster were observed. The restriction profile clusters in the 5'UTR corresponded to the phylogenetic clusters in the VP1 genomic region. The incongruence between the topology of Gior strain in 5'UTR and VP1 phylogenetic trees indicates a recombination event. The proposed RFLP assay in combination with VP1 sequencing can offer crucial epidemiological information about the circulating enteroviruses.
肠道病毒是无菌性脑膜炎的主要病因，由 100 种血清型组成，其中许多与大规模爆发有关。在本研究中，对 27 株临床肠道病毒株的 5'非翻译区（5'UTR）的 RFLP 分析和 5'UTR 和 VP1 区的序列分析进行了比较，以进行流行病学关联。临床肠道病毒株聚类为五个限制谱群。尽管临床分离株的限制谱群与相应原型株的限制谱群无关，但观察到每个聚类成员之间存在流行病学关系。5'UTR 中的限制谱群与 VP1 基因组区域的系统发育聚类相对应。Gior 株在 5'UTR 和 VP1 系统发育树中的拓扑结构不一致表明存在重组事件。该 RFLP 检测与 VP1 测序相结合，可为循环肠道病毒提供重要的流行病学信息。
