Department of Infectious Diseases/Section of Clinical Virology, Institute of Biomedicine, University of Gothenburg, Gothenburg, Sweden.
J Clin Virol. 2013 Sep;58(1):227-32. doi: 10.1016/j.jcv.2013.07.005. Epub 2013 Jul 27.
Infections caused by human enteroviruses (EVs) are often asymptomatic or mild, although they may cause more severe illnesses as meningitis and acute flaccid paralysis. EVs have globally posed a threat to children, and outbreaks of aseptic meningitis and hand, foot and mouth disease are frequently reported.
To identify EV strains circulating among healthy children in a small community in Limbe, Cameroon two years apart.
Species and EV types were obtained by partial 5'UTR-VP4 and VP1 sequencing of RNA from stool samples collected in October 2009 and September 2011 from 150 children in Cameroon.
In all, 74 children (49%) were infected with 28 different types of EV. There were 29 (54%) infected children in 2009, and 45 (47%) in 2011. There was a significant difference between detected species of EV, with 15 (47%) children infected with EV-A in 2009, and 22 (71%) with EV-B in 2011 (p=0.0001). In 2009, one child was infected by a divergent EV, which was most similar to EV-A90. Based on the complete VP1 sequence, it was shown to be a new EV designated EV-A119.
The current study shows a high heterogeneity of circulating EV types among children in Limbe, Cameroon, and a previously not described shift in predominating EV species.
人类肠道病毒（EVs）引起的感染通常是无症状或轻症的，但它们也可能引起更严重的疾病，如脑膜炎和急性弛缓性麻痹。EVs 在全球范围内对儿童构成威胁，无菌性脑膜炎和手足口病的爆发经常发生。
在喀麦隆利姆贝的一个小社区中，相隔两年确定健康儿童中循环的 EV 株。
通过对 2009 年 10 月和 2011 年 9 月采集的 150 名喀麦隆儿童粪便样本中的 RNA 进行部分 5'UTR-VP4 和 VP1 测序，获得了种系和 EV 型。
共有 74 名儿童（49%）感染了 28 种不同类型的 EV。2009 年有 29 名（54%）感染儿童，2011 年有 45 名（47%）。EV 检测到的种系有显著差异，2009 年有 15 名（47%）儿童感染 EV-A，2011 年有 22 名（71%）儿童感染 EV-B（p=0.0001）。2009 年，一名儿童感染了一种与其他 EV 不同的 EV，与 EV-A90 最为相似。根据完整的 VP1 序列，表明它是一种新的 EV，被命名为 EV-A119。
本研究表明，喀麦隆利姆贝儿童中循环的 EV 型具有高度异质性，并且优势 EV 种系发生了以前未描述的变化。
