Department of Bioengineering, University of Texas at Arlington, Arlington, Texas, USA.
Lab Chip. 2012 Nov 21;12(22):4693-701. doi: 10.1039/c2lc21251j.
Early detection and isolation of circulating tumor cells (CTC) can enable better prognosis for cancer patients. A Hele-Shaw device with aptamer functionalized glass beads is designed, modeled, and fabricated to efficiently isolate cancer cells from a cellular mixture. The glass beads are functionalized with anti-epidermal growth factor receptor (EGFR) aptamer and sit in ordered array of pits in polydimethylsiloxane (PDMS) channel. A PDMS encapsulation is then used to cover the channel and to flow through cell solution. The beads capture cancer cells from flowing solution depicting high selectivity. The cell-bound glass beads are then re-suspended from the device surface followed by the release of 92% cells from glass beads using combination of soft shaking and anti-sense RNA. This approach ensures that the cells remain in native state and undisturbed during capture, isolation and elution for post-analysis. The use of highly selective anti-EGFR aptamer with the glass beads in an array and subsequent release of cells with antisense molecules provide multiple levels of binding and release opportunities that can help in defining new classes of CTC enumeration devices.
早期发现和隔离循环肿瘤细胞（CTC）可以为癌症患者带来更好的预后。设计、建模和制造了一种带有适体功能化玻璃珠的Hele-Shaw 装置，以有效地从细胞混合物中分离癌细胞。玻璃珠用抗表皮生长因子受体（EGFR）适体功能化，并位于 PDMS 通道中的有序排列的凹坑中。然后使用 PDMS 封装覆盖通道并流经细胞溶液。珠子从流动的溶液中捕获癌细胞，表现出高选择性。然后将细胞结合的玻璃珠从装置表面重新悬浮，然后使用软摇床和反义 RNA 的组合从玻璃珠上释放 92%的细胞。这种方法确保在捕获、分离和洗脱进行后续分析过程中，细胞保持天然状态且不受干扰。在阵列中使用高度选择性的抗 EGFR 适体与玻璃珠，并随后使用反义分子释放细胞，提供了多个结合和释放机会，有助于定义新类别的 CTC 计数装置。
