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三步流聚焦实现了基于图像的晚期雨生红球藻细胞的鉴别和分选。

Three step flow focusing enables image-based discrimination and sorting of late stage 1 Haematococcus pluvialis cells.

机构信息

Leibniz Institute of Photonic Technology, Jena, Germany.

Gesellschaft zur Förderung von Medizin-, Bio- und Umwelttechnologien e. V. (GMBU), Halle (Saale), Germany.

出版信息

PLoS One. 2021 Mar 29;16(3):e0249192. doi: 10.1371/journal.pone.0249192. eCollection 2021.

DOI:10.1371/journal.pone.0249192
PMID:33780476
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8007022/
Abstract

Label-free and gentle separation of cell stages with desired target properties from mixed stage populations are a major research task in modern biotechnological cultivation process and optimization of micro algae. The reported microfluidic sorter system (MSS) allows the subsequent investigation of separated subpopulations. The implementation of a viability preserving MSS is shown for separation of late stage 1 Haematococcus pluvialis (HP) cells form a mixed stage population. The MSS combines a three-step flow focusing unit for aligning the cells in single file transportation mode at the center of the microfluidic channel with a pure hydrodynamic sorter structure for cell sorting. Lateral displacement of the cells into one of the two outlet channels is generated by piezo-actuated pump chambers. In-line decision making for sorting is based on a user-definable set of image features and properties. The reported MSS significantly increased the purity of target cells in the sorted population (94%) in comparison to the initial mixed stage population (19%).

摘要

无标记且温和地从混合阶段群体中分离具有所需目标特性的细胞阶段是现代生物技术培养过程和优化微藻的主要研究任务。所报道的微流控分选系统 (MSS) 允许对分离的亚群进行后续研究。为了从混合阶段的血球藻 (HP) 细胞群体中分离晚期 1 期的 HP 细胞，展示了一种具有生存能力的 MSS 的实施。MSS 将用于将细胞以单排运输模式在微流道中心对齐的三步流聚焦单元与用于细胞分选的纯流体动力分选结构相结合。通过压电驱动的泵室将细胞侧向移位到两个出口通道之一中。基于用户定义的一组图像特征和属性，在线进行分选决策。与初始混合阶段群体 (19%) 相比，所报道的 MSS 显著提高了分选群体中目标细胞的纯度 (94%)。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adc4/8007022/4201c99f6157/pone.0249192.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adc4/8007022/d70c31387e97/pone.0249192.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adc4/8007022/c4d3add02116/pone.0249192.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adc4/8007022/fa669087e01e/pone.0249192.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adc4/8007022/bd374f5e9160/pone.0249192.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adc4/8007022/861ac01ee5a0/pone.0249192.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adc4/8007022/4201c99f6157/pone.0249192.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adc4/8007022/d70c31387e97/pone.0249192.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adc4/8007022/c4d3add02116/pone.0249192.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adc4/8007022/fa669087e01e/pone.0249192.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adc4/8007022/bd374f5e9160/pone.0249192.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adc4/8007022/861ac01ee5a0/pone.0249192.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adc4/8007022/4201c99f6157/pone.0249192.g006.jpg

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