NSF NSEC Center for High-Rate Nanomanufacturing, Northeastern University, Boston, MA 02115, USA.
Lab Chip. 2012 Nov 21;12(22):4748-54. doi: 10.1039/c2lc40580f.
This paper describes a microscale in vivo sensor platform device for the simultaneous detection of multiple biomarkers. We designed the polymer-based biosensors incorporating multiple active isolated areas, as small as 70 μm × 70 μm, for antigen detection. The fabrication approach involved conventional micro- and nano-fabrication processes followed by site-specific electrophoretic directed assembly of antibody-functionalized nanoparticles. To ensure precise and large-scale manufacturing of these biosensors, we developed a semi-automated system for the attachment of the 250-μm biosensor to a 300-μm catheter probe. Our fabrication and post-processing procedures should enable large-scale production of such biosensor devices at lower manufacturing cost. The principle of detection with these biosensors involved a simple fluorescence-based enzyme-linked immunosorbent assay. These biosensors exhibit high selectivity (ability to selectively detect multiple biomarkers of different diseases), specificity (ability to target generic to specific disease biomarkers), rapid antigen uptake, and low detection limits (for carcinoembryonic antigen, 31.25 pg mL(-1); for nucleosomes, 62.5 pg mL(-1)), laying the foundation for potential early detection of various diseases.
本文描述了一种用于同时检测多种生物标志物的微尺度体内传感器平台设备。我们设计了基于聚合物的生物传感器，包含多个活性隔离区域，其大小小至 70μm×70μm，用于抗原检测。该制造方法涉及传统的微纳加工工艺，然后通过特异性电泳定向组装抗体功能化纳米颗粒。为了确保这些生物传感器的精确和大规模制造，我们开发了一种半自动系统，用于将 250μm 的生物传感器附着到 300μm 的导管探针上。我们的制造和后处理程序应该能够以更低的制造成本大规模生产这种生物传感器设备。这些生物传感器的检测原理涉及一种简单的基于荧光的酶联免疫吸附测定法。这些生物传感器表现出高选择性（能够选择性地检测来自不同疾病的多种生物标志物）、特异性（能够针对通用到特定疾病的生物标志物）、快速的抗原摄取和低检测限（对癌胚抗原为 31.25pgmL(-1)；对核小体为 62.5pgmL(-1)），为各种疾病的早期检测奠定了基础。
