Inter University Centre for Genomics and Gene Technology, University of Kerala, Kariavattom Campus, Thiruvananthapuram, Kerala 695 581, India.
Appl Microbiol Biotechnol. 2013 Jun;97(12):5615-25. doi: 10.1007/s00253-012-4389-1. Epub 2012 Sep 18.
Microorganisms tend to colonize on solid metal/alloy surface in natural environment leading to loss of utility. Microbiologically influenced corrosion or biocorrosion usually increases the corrosion rate of steel articles due to the presence of bacteria that accelerates the anodic and/or cathodic corrosion reaction rate without any significant change in the corrosion mechanism. An attempt was made in the present study to protect hot-dip galvanized steel from such attack of biocorrosion by means of chemically modifying the zinc coating. W-TiO2 composite was synthesized and incorporated into the zinc bath during the hot-dipping process. The surface morphology and elemental composition of the hot-dip galvanized coupons were analyzed by scanning electron microscopy and energy dispersive X-ray spectroscopy. The antifouling characteristics of the coatings were analyzed in three different solutions including distilled water, seawater, and seawater containing biofilm scrapings under immersed conditions. Apart from electrochemical studies, the biocidal effect of the composite was evaluated by analyzing the extent of bacterial growth due to the presence and absence of the composite based on the analysis of total extracellular polymeric substance and total biomass using microtiter plate assay. The biofilm-forming bacteria formed on the surface of the coatings was cultured on Zobell Marine Agar plates and studied. The composite was found to be effective in controlling the growth of bacteria and formation of biofilm thereafter.
微生物倾向于在自然环境中的固体金属/合金表面定殖，导致其失去效用。由于存在加速阳极和/或阴极腐蚀反应速率而不改变腐蚀机制的细菌，微生物影响的腐蚀或生物腐蚀通常会增加钢制品的腐蚀速率。本研究试图通过化学修饰锌涂层来保护热浸镀锌钢免受这种生物腐蚀的侵害。W-TiO2 复合材料被合成并在热浸镀过程中加入锌浴中。通过扫描电子显微镜和能量色散 X 射线能谱分析了热浸镀锌试件的表面形貌和元素组成。在浸入条件下，在三种不同的溶液中（包括蒸馏水、海水和含有生物膜刮屑的海水）分析了涂层的防污特性。除了电化学研究外，还通过分析总胞外聚合物物质和总生物量的分析，基于存在和不存在基于复合材料的分析，评估了复合材料的杀菌效果，使用微量滴定板测定法。在涂层表面形成的生物膜形成细菌在 Zobell 海洋琼脂平板上培养并进行了研究。发现该复合材料能有效控制细菌的生长和随后生物膜的形成。
