Biochemical Sciences Division, National Chemical Laboratory, Pune 411008, India.
Appl Biochem Biotechnol. 2012 Dec;168(7):1806-19. doi: 10.1007/s12010-012-9898-y. Epub 2012 Sep 16.
An effective alkali pretreatment which affects the structural properties of cellulose (corn cob) has been studied. The pretreatment of corn cob was carried out with different combinations of alkali at varying temperatures. The most effective pretreatment of corn cob was achieved with 1 % alkali at 50 °C in 4 h. The crystallinity index (CrI) and specific surface area (SSA) of untreated corn cob was 39 % and 0.52 m(2)/g wherein after alkali pretreatment CrI decreased to 15 % and SSA increased to 3.32 m(2)/g. The fungal organism was identified as Penicillium pinophilum on the basis of ITS sequence. At 5 % substrate concentration using a complete cellulase from Penicillium pinophilum the hydrolysis of untreated corn cob with 5, 10 and 20 FPU/g enzyme loadings were 11 %, 13 % and 16 %, whereas after alkali treatment the hydrolysis increased to 78 %, 90 % and 100 %, respectively. Further hydrolytic potential of commercial cellulases viz. Accellerase™ 1,000, Palkofeel-30 and Palkocel-40 were investigated under similar conditions.
已经研究了一种有效的碱预处理方法，该方法会影响纤维素（玉米芯）的结构特性。采用不同碱组合在不同温度下对玉米芯进行预处理。在 50°C 下用 1%的碱处理 4 小时，对玉米芯的预处理效果最佳。未经处理的玉米芯的结晶度指数（CrI）和比表面积（SSA）分别为 39%和 0.52 m²/g，而经过碱预处理后，CrI 降低至 15%，SSA 增加至 3.32 m²/g。基于 ITS 序列，将真菌鉴定为嗜松青霉。在 5%的底物浓度下，使用来自嗜松青霉的完整纤维素酶，未经过碱处理的玉米芯在 5、10 和 20 FPU/g 酶负荷下的水解率分别为 11%、13%和 16%，而经过碱处理后，水解率分别增加至 78%、90%和 100%。在类似条件下，还研究了商业纤维素酶 Accellerase™ 1,000、Palkofeel-30 和 Palkocel-40 的进一步水解潜力。
