Department of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, #41 Damucang xidan, Beijing, 100032, China.
Clin Exp Med. 2013 Nov;13(4):245-50. doi: 10.1007/s10238-012-0207-4. Epub 2012 Sep 15.
Clinical significance of anti-Saccharomyces cerevisiae antibody (ASCA) and its prevalence in Chinese primary biliary cirrhosis (PBC) patients have not been characterized and therefore needs to be defined. Enzyme-linked immunosorbent assay was used to test ASCA in sera from 198 PBC patients, 85 patients with other liver diseases (OLD) and 35 health controls (HC). Indirect immunofluorescence was used to detect anti-mitochondrial antibodies (AMA) in PBC. Results showed that the frequency of ASCA in PBC, 29.8 %, was higher than other disease groups. And ASCA occurred more frequently in PBC patients with positive anti-gp210 than the negative ones. Also, ASCA was detected in 7 out of 15 PBC negative for AMA. Some liver-related biochemical indices and inflammatory indices were significantly higher in PBC patients with positive ASCA (p < 0.05). In conclusion, the prevalence of ASCA in Chinese PBC patients is 29.8 %. PBC patients with positive ASCA are associated with more severe liver injury, and ASCA-IgA might be related to disease activity of PBC.
抗酿酒酵母抗体（ASCA）在中国人原发性胆汁性胆管炎（PBC）患者中的临床意义及其流行率尚未确定，因此需要加以定义。我们采用酶联免疫吸附试验（ELISA）检测了 198 例 PBC 患者、85 例其他肝病（OLD）患者和 35 名健康对照者（HC）的血清 ASCA。采用间接免疫荧光法（IFA）检测 PBC 中的抗线粒体抗体（AMA）。结果显示，PBC 患者中 ASCA 的频率为 29.8%，高于其他疾病组。并且，在抗 gp210 阳性的 PBC 患者中，ASCA 的发生率高于阴性患者。此外，在 15 例 AMA 阴性的 PBC 患者中也检测到了 ASCA。阳性 ASCA 的 PBC 患者的一些与肝脏相关的生化指标和炎症指标显著升高（p<0.05）。总之，中国 PBC 患者中 ASCA 的流行率为 29.8%。阳性 ASCA 的 PBC 患者与更严重的肝损伤相关，并且 ASCA-IgA 可能与 PBC 的疾病活动度有关。
