米卡芬净和卡泊芬净对中国分离的皮肤癣菌的体外抗真菌活性。
In vitro antifungal activity of micafungin and caspofungin against dermatophytes isolated from China.
机构信息
Department of Dermatology, Peking University First Hospital, Research Center for Medical Mycology, Peking University, No. 8 Xishiku St., Xicheng District, Beijing, 100034, China.
出版信息
Mycopathologia. 2013 Feb;175(1-2):141-5. doi: 10.1007/s11046-012-9571-6. Epub 2012 Sep 17.
AIMS
The aims of this study are to investigate the in vitro activities of micafungin and caspofungin that are two new echinocandin antifungal drugs against clinically isolated dermatophytes in China and to define MEC (minimal effective concentration) as the reading endpoints of this study in accordance with (Clinical and laboratory Standards Institute) CLSI M38-A2 reference.
METHODS
Minimal effective concentrations (MECs) of micafungin and caspofungin for 82 dermatophyte strains were determined according to CLSI (formerly NCCLS) M38-A2 broth microdilution methods.
RESULTS
(1) The MEC(90s) of micafungin for Trichophyton violaceum and Trichophyton tonsurans were 0.25 μg/mL, and for Microsporum canis and Trichophyton verrucosum were 0.06 μg/mL. The MEC(90s) for Trichophyton rubrum, Trichophyton mentagrophytes, Microsporum gypseum and Epidermophyton floccosum were 0.03 μg/mL. (2) The MEC(90s) of caspofungin for T. rubrum, T. violaceum and T. tonsurans were 1 μg/mL, and for T. mentagrophytes, M. canis, M. gypseum, E. floccosum and T. verrucosum were 0.5 μg/mL. (3) Compared with caspofungin, micafungin demonstrated lower MEC value to dermatophytes (P < 0.05).
CONCLUSIONS
Micafungin has stronger in vitro antifungal activity than caspofungin.
目的
本研究旨在研究两种新型棘白菌素类抗真菌药物米卡芬净和卡泊芬净对中国临床分离的皮肤癣菌的体外活性，并根据（临床和实验室标准协会）CLSI M38-A2 参考标准，将最小有效浓度（MEC）定义为该研究的读数终点。
方法
根据 CLSI（前身为 NCCLS）M38-A2 肉汤微量稀释法，测定 82 株皮肤癣菌对米卡芬净和卡泊芬净的最小有效浓度（MEC）。
结果
（1）米卡芬净对紫色毛癣菌和须癣毛癣菌的 MEC（90s）为 0.25μg/mL，对犬小孢子菌和疣状毛癣菌的 MEC（90s）为 0.06μg/mL。红色毛癣菌、须癣毛癣菌、石膏样小孢子菌和絮状表皮癣菌的 MEC（90s）为 0.03μg/mL。（2）卡泊芬净对红色毛癣菌、紫色毛癣菌和须癣毛癣菌的 MEC（90s）为 1μg/mL，对絮状表皮癣菌、犬小孢子菌、石膏样小孢子菌和毛癣菌的 MEC（90s）为 0.5μg/mL。（3）与卡泊芬净相比，米卡芬净对皮肤癣菌的 MEC 值较低（P<0.05）。
结论
米卡芬净对皮肤癣菌具有比卡泊芬净更强的体外抗真菌活性。
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