Song Erqun, Xu Li
Laboratory of Luminescence and Real-Time Analysis, Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing, China, 400715.
J Chromatogr Sci. 2013 Apr;51(4):331-4. doi: 10.1093/chromsci/bms144. Epub 2012 Sep 14.
In the present study, a rapid fingerprint analysis of Ligusticum chuanxiong (L. chuanxiong) was developed based on ultra-fast liquid chromatography coupled with diode array detection (UFLC-DAD). The analysis time was shortened from approximately 75 min on conventional high-performance liquid chromatography (HPLC) to 40 min on UFLC. This method was validated in terms of stability [<4.40% relative standard deviation (RSD)], precision (<4.26% RSD) and repeatability (<2.82% RSD). Six batches of L. chuanxiong from different sources were analyzed by UFLC-DAD, and the results were systematically processed using professional analytical software that was recommended by the State Food and Drug Administration of China. The similarities of these six batches of samples were evaluated. Compared to conventional HPLC, the UFLC-DAD method was fast and sensitive and consumed less solvent, and is widely applicable for quality monitoring of traditional Chinese medicine.
在本研究中，基于超快速液相色谱-二极管阵列检测联用技术（UFLC-DAD）建立了川芎的快速指纹图谱分析方法。分析时间从传统高效液相色谱（HPLC）的约75分钟缩短至UFLC的40分钟。该方法在稳定性[相对标准偏差（RSD）<4.40%]、精密度（RSD<4.26%）和重复性（RSD<2.82%）方面得到了验证。采用UFLC-DAD对6批不同来源的川芎进行分析，并使用中国国家食品药品监督管理总局推荐的专业分析软件对结果进行系统处理。对这6批样品的相似度进行了评价。与传统HPLC相比，UFLC-DAD方法快速、灵敏，溶剂消耗少，广泛适用于中药质量监测。
