关于花菁荧光团光稳定性的机制
On the Mechanisms of Cyanine Fluorophore Photostabilization.
作者信息
Zheng Qinsi, Jockusch Steffen, Zhou Zhou, Altman Roger B, Warren J David, Turro Nicholas J, Blanchard Scott C
机构信息
Department of Physiology and Biophysics, Weill Medical College of Cornell University, 1300 York Avenue, New York, New York 10065, United States ; Tri-Institutional Training Program in Chemical Biology, Weill Medical College of Cornell University, 1300 York Avenue, New York, New York 10065, United States.
出版信息
J Phys Chem Lett. 2012 Aug 16;3(16):2200-2203. doi: 10.1021/jz300670p. Epub 2012 Jul 30.
Cyanine fluorophores exhibit greatly improved photostability when covalently linked to stabilizers, such as cyclooctatetraene (COT), nitrobenzyl alcohol (NBA) or Trolox. However, the mechanism by which photostabilization is mediated has yet to be determined. Here we present spectroscopic evidence that COT, when covalently linked to Cy5, substantially reduces the lifetime of the Cy5 triplet state, and that the degree of triplet state quenching correlates with enhancements in photostability observed in single-molecule fluorescence measurements. By contrast, NBA and Trolox did not quench the Cy5 triplet state under our conditions suggesting that their mechanism of photostabilization is different from COT and does not target the fluorophore triplet state directly. These findings provide insights into the mechanisms of fluorophore photostabilization that may lead to improved fluorophore designs for biological imaging applications.
当与诸如环辛四烯（COT）、硝基苯甲醇（NBA）或生育三烯酚（Trolox）等稳定剂共价连接时，花菁荧光团表现出显著提高的光稳定性。然而，光稳定化介导的机制尚未确定。在这里，我们提供光谱证据表明，当COT与Cy5共价连接时，会显著缩短Cy5三重态的寿命，并且三重态猝灭程度与单分子荧光测量中观察到的光稳定性增强相关。相比之下，在我们的条件下，NBA和Trolox不会猝灭Cy5三重态，这表明它们的光稳定化机制与COT不同，并且不会直接靶向荧光团三重态。这些发现为荧光团光稳定化机制提供了见解，这可能会导致用于生物成像应用的改进荧光团设计。
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